MIF (Macrophage Inhibitory Factor; also GIF) is a 12-13 kDa member of the MIF family of proteins. Although secreted, it possesses no definitive signal sequence. MIF is widely expressed and serves to regulate immune reactions. On macrophages and B cells, MIF binds to a CD74:CD44 complex, initiating downstream signaling. On monocytes, this promotes TNF alpha production plus IFN-gamma -stimulated NO production. On B cells, this promotes B cell survival. MIF also binds to leukocyte CD74:CXCR2 and CD74:CXCR4 heterodimers, initiating T cell and monocyte migration. A tautomerase activity has also been shown for MIF and is suggested to involve the entire length of the molecule. Mouse MIF is 114 amino acids (aa) in length. MIF is phosphorylated at Ser91, and an additional, singular Cys residue is covalently linked to MIF at Cys60. MIF is considered to act as a homotrimer. Full-length mouse MIF shows 90% and 99% aa sequence identity with human and rat MIF, respectively.
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgM Clone # 811409
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Product Specifications
Immunogen
E. coli-derived recombinant mouse MIF
Met1-Ala115
Accession # P34884
Met1-Ala115
Accession # P34884
Specificity
Detects mouse MIF in ELISA and Western Blot.
Clonality
Monoclonal
Host
Rat
Isotype
IgM
Scientific Data Images for Mouse/Rat MIF Antibody
Detection of Mouse MIF by Western Blot.
Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB19781) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat MIF by Western Blot.
Western blot shows lysates of NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB19781) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of MIF in J774A.1 Mouse Cell Line by Flow Cytometry.
J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB19781, filled histogram) or rat IgM isotype control antibody, followed by Allophycocyanin-conjugated anti-rat IgM. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Mouse/Rat MIF Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MIF
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
UniProt
Additional MIF Products
Product Documents for Mouse/Rat MIF Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat MIF Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Protocol
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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