Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

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Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve
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Product Details
Citations (5)
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Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (50 uL), Mouse Serum (10 uL), Rat Serum (10 uL), Canine Serum (10 uL), Porcine Serum (10 uL)
1.87 pg/mL
Assay Range
12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Mouse Serum, Rat Serum, Canine Serum, Porcine Serum)
Natural and recombinant IGF-II.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine® Mouse/Rat/Porcine/Canine IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure IGF-II in serum-free cell culture supernates and serum. It contains E. coli-expressed recombinant IGF-II and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural IGF-II.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum-free Cell Culture Supernates, Serum, Mouse Serum, Rat Serum, Canine Serum, Porcine Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 46.1 81.7 285 43.5 80.7 275
Standard Deviation 2.14 3.41 10.8 2.58 4.97 16.8
CV% 4.6 4.2 3.8 5.9 6.2 6.1


The recovery of IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Serum (n=4) 105 86-124
Rat Serum (n=4) 84 78-89
Serum-free Cell Culture Media (n=4) 92 84-101


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat/Porcine/Canine IGF-II Linearity

Scientific Data

Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-II/IGF2

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

Long Name:
Insulin-like Growth Factor II
Entrez Gene IDs:
3481 (Human); 16002 (Mouse)
Alternate Names:
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide and methanol, which are known to the State of California to cause cancer and reproductive toxicity with developmental effects. For more information, go to

Citations for Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production
    Authors: K Yamanaka, Y Haraguchi, H Takahashi, I Kawashima, T Shimizu
    Scientific Reports, 2023-01-10;13(1):498.
    Species: Bovine
    Sample Types: Cell Culture Supernates.
  2. Comparative Effects of Allulose, Fructose, and Glucose on the Small Intestine
    Authors: T Suzuki, Y Sato, S Kadoya, T Takahashi, M Otomo, H Kobayashi, K Aoki, M Kantake, M Sugiyama, RP Ferraris
    Nutrients, 2022-08-07;14(15):.
    Species: Rat
    Sample Types: Plasma
  3. Critical timing of ACEi initiation prevents compensatory glomerular hypertrophy in the remaining single kidney
    Authors: AS Naik, SQ Wang, M Chowdhury, J Aqeel, CL O'Connor, JE Wiggins, M Bitzer, RC Wiggins
    Scientific Reports, 2021-10-01;11(1):19605.
    Species: Rat
    Sample Types: Urine
  4. Runx2+ Niche Cells Maintain Incisor Mesenchymal Tissue Homeostasis through IGF Signaling
    Authors: S Chen, J Jing, Y Yuan, J Feng, X Han, Q Wen, TV Ho, C Lee, Y Chai
    Cell Rep, 2020-08-11;32(6):108007.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice
    Authors: Y Sui, W Zhang, T Tang, L Gao, T Cao, H Zhu, Q You, B Yu, T Yang
    Stem Cell Res Ther, 2020-02-26;11(1):86.
    Species: Mouse
    Sample Types: Serum


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