Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

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MG200
Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve
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Citations (3)
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Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Serum-free Cell Culture Supernates (50 uL), Mouse Serum (10 uL), Rat Serum (10 uL), Canine Serum (10 uL), Porcine Serum (10 uL)
Sensitivity
1.87 pg/mL
Assay Range
12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Mouse Serum, Rat Serum, Canine Serum, Porcine Serum)
Specificity
Natural and recombinant IGF-II.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine® Mouse/Rat/Porcine/Canine IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure IGF-II in serum-free cell culture supernates and serum. It contains E. coli-expressed recombinant IGF-II and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural IGF-II.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum-free Cell Culture Supernates, Serum, Mouse Serum, Rat Serum, Canine Serum, Porcine Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 46.1 81.7 285 43.5 80.7 275
Standard Deviation 2.14 3.41 10.8 2.58 4.97 16.8
CV% 4.6 4.2 3.8 5.9 6.2 6.1

Recovery

The recovery of IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Serum (n=4) 105 86-124
Rat Serum (n=4) 84 78-89
Serum-free Cell Culture Media (n=4) 92 84-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat/Porcine/Canine IGF-II Linearity

Data Examples

Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-II/IGF2

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

Long Name:
Insulin-like Growth Factor II
Entrez Gene IDs:
3481 (Human); 16002 (Mouse)
Alternate Names:
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide and methanol, which are known to the State of California to cause cancer and reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Critical timing of ACEi initiation prevents compensatory glomerular hypertrophy in the remaining single kidney
    Authors: AS Naik, SQ Wang, M Chowdhury, J Aqeel, CL O'Connor, JE Wiggins, M Bitzer, RC Wiggins
    Scientific Reports, 2021;11(1):19605.
    Species: Rat
    Sample Types: Urine
  2. Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice
    Authors: Y Sui, W Zhang, T Tang, L Gao, T Cao, H Zhu, Q You, B Yu, T Yang
    Stem Cell Res Ther, 2020;11(1):86.
    Species: Mouse
    Sample Types: Serum
  3. Runx2+ Niche Cells Maintain Incisor Mesenchymal Tissue Homeostasis through IGF Signaling
    Authors: S Chen, J Jing, Y Yuan, J Feng, X Han, Q Wen, TV Ho, C Lee, Y Chai
    Cell Rep, 2020;32(6):108007.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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