Mouse/Rat RIBP/SH2D2A Alexa Fluor® 647-conjugated Antibody

Catalog #: AF6265R Datasheet / COA / SDS

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AF6265R-100UG

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Mouse/Rat RIBP/SH2D2A Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat RIBP/SH2D2A in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse RIBP/SH2D2A
Ile299-Ser374
Accession # Q9QXK9

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SH2D2A

RIBP (Rlk/Itk‑binding protein; also SH2D2A, LAD/Lck‑associated adapter protein, MEKK2‑binding protein and TSAd) is a 45‑47 kDa cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR‑initiated proliferation, plus IL‑2 and IFN‑ gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374 amino acids (aa) in length and contains one SH2 domain (aa 116‑207), plus a Pro‑rich region (aa 267‑308) that contains an SH3‑binding motif (aa 267‑273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa 254‑261. Over aa 299‑374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively.

Entrez Gene IDs

9047 (Human); 27371 (Mouse); 310688 (Rat)

Alternate Names

F2771; RIBP; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; SH2D2A; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAd; TSADSH2 domain-containing protein 2A; VEGF receptor-associated protein; VRAP