Mouse/Rat RIBP/SH2D2A Antibody

Catalog # Availability Size / Price Qty
AF6265
AF6265-SP
Mouse/Rat RIBP/SH2D2A Antibody in Western Blot
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse/Rat RIBP/SH2D2A Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat RIBP/SH2D2A in Western blots. 
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse RIBP/SH2D2A
Ile299-Ser374
Accession # Q9QXK9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse and Rat RIBP/SH2D2A by Western Blot. View Larger

Detection of Mouse and Rat RIBP/SH2D2A by Western Blot. Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line and Nb2-11 rat lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat RIBP/SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIBP/SH2D2A at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2D2A

RIBP (Rlk/Itk‑binding protein; also SH2D2A, LAD/Lck‑associated adapter protein, MEKK2‑binding protein and TSAd) is a 45‑47 kDa cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR‑initiated proliferation, plus IL‑2 and IFN‑ gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374 amino acids (aa) in length and contains one SH2 domain (aa 116‑207), plus a Pro‑rich region (aa 267‑308) that contains an SH3‑binding motif (aa 267‑273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa 254‑261. Over aa 299‑374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively.

Entrez Gene IDs
9047 (Human); 27371 (Mouse); 310688 (Rat)
Alternate Names
F2771; RIBP; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; SH2D2A; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAd; TSADSH2 domain-containing protein 2A; VEGF receptor-associated protein; VRAP

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse/Rat RIBP/SH2D2A Antibody

There are currently no reviews for this product. Be the first to review Mouse/Rat RIBP/SH2D2A Antibody and earn rewards!

Have you used Mouse/Rat RIBP/SH2D2A Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review