Detection of Mouse and Rat RIBP/SH2D2A by Western Blot.
Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line and Nb2‑11 rat lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat RIBP/SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIBP/SH2D2A at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
RIBP (Rlk/Itk‑binding protein; also SH2D2A, LAD/Lck‑associated adapter protein, MEKK2‑binding protein and TSAd) is a 45‑47 kDa cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR‑initiated proliferation, plus IL‑2 and IFN‑ gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374 amino acids (aa) in length and contains one SH2 domain (aa 116‑207), plus a Pro‑rich region (aa 267‑308) that contains an SH3‑binding motif (aa 267‑273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa 254‑261. Over aa 299‑374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively.
Entrez Gene IDs:
9047 (Human); 27371 (Mouse); 310688 (Rat)
F2771; RIBP; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAd; TSADSH2 domain-containing protein 2A; VEGF receptor-associated protein; VRAP
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