Mouse RGM-A Biotinylated Antibody

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Mouse RGM-A Biotinylated Antibody Summary

Species Reactivity
Detects mouse RGM-A in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human RGM-A is observed and 5% cross-reactivity with recombinant chicken RGM is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse RGM-A
Accession # Q6PCX7
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Repulsive Guidance Molecule A/RGM-A (Catalog # 2458-RG)
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

Mouse repulsive guidance molecule (RGM-A) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 454 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 122 aa N-terminal prosegment, a 258 mature region and a 27 aa C-terminal prosegment (2, 3). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of mouse RGM-A is 93% and 87% aa identical to human and chick mature region RGM-A, respectively. When compared to mouse RGMb and c, the mature region of mouse RGM-A shows 77% and 76% aa identity, respectively (2, 3). Recombinant chick RGM has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mice, however, this activity is not so obvious, and the function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure (3), while chick RGM-A is reported to be responsible for the layered segmentation of entorhinal cortical projections to the hippocampus (4). The receptor for RGM is reported to be neogenin (5, 6).

  1. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  2. Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
  3. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  4. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  5. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  6. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
Long Name
Repulsive Guidance Molecule A
Entrez Gene IDs
56963 (Human); 244058 (Mouse)
Alternate Names
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

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