Mouse RGM-A Antibody

• Species Reactivity
  Mouse
• Specificity
  Detects mouse RGM-A in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human RGM-A and recombinant chicken RGM-A is observed and less than 1% cross-reactivity with recombinant mouse (rm) RGM-B and rmRGM-C is observed.
  
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant mouse RGM-A
  Cys48-Gly421
  Accession # Q6PCX7
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.1 µg/mL
  Recombinant Mouse Repulsive Guidance Molecule A/RGM-A (Catalog # 2458-RG)
• Immunohistochemistry
  5-15 µg/mL
  See below

Immunohistochemistry click image to view larger

RGM‑A in Mouse Embryo. RGM‑A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c) using Goat Anti-Mouse RGM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2458) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to retina and developing neurons in cross-section of the developing eye. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  2. Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
  3. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  4. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  5. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  6. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.

• Long Name:
  Repulsive Guidance Molecule A

• Entrez Gene IDs:
  56963 (Human); 244058 (Mouse)

• Alternate Names:
  RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

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