Detects mouse RGM-A in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human RGM-A and recombinant chicken RGM-A is observed and less than 1% cross-reactivity with recombinant mouse (rm) RGM-B and rmRGM-C is observed.
RGM‑A in Mouse Embryo.
RGM‑A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c) using Goat Anti-Mouse RGM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2458) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to retina and developing neurons in cross-section of the developing eye. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse repulsive guidance molecule (RGM-A) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 454 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 122 aa N-terminal prosegment, a 258 mature region and a 27 aa C-terminal prosegment (2, 3). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of mouse RGM-A is 93% and 87% aa identical to human and chick mature region RGM-A, respectively. When compared to mouse RGMb and c, the mature region of mouse RGM-A shows 77% and 76% aa identity, respectively (2, 3). Recombinant chick RGM has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mice, however, this activity is not so obvious, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure (3). And in a mouse culture system, chick RGM-A is reported to be responsible for the layered segmentation of of entorhinal cortical projections to the hippocampus (4). The receptor for RGM is reported to be neogenin (5, 6).
Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
Niederkofler V. et al. (2004) J. Neurosci. 24:808.
Brinks, H. et al. (2004) J. Neurosci. 24:3862.
Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
Repulsive Guidance Molecule A
Entrez Gene IDs:
56963 (Human); 244058 (Mouse)
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A
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The data collected includes not only links to publications in PubMed,
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