Mouse RGM-A Antibody

AF2458 1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2458
AF2458-SP

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Mouse RGM-A Antibody in Immunohistochemistry

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse RGM-A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse RGM-A in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human RGM-A and recombinant chicken RGM-A is observed and less than 1% cross-reactivity with recombinant mouse (rm) RGM-B and rmRGM-C is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RGM-A
Cys48-Gly421
Accession # Q6PCX7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Repulsive Guidance Molecule A/RGM-A (Catalog # 2458-RG)

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry

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RGM‑A in Mouse Embryo.
RGM‑A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c) using Goat Anti-Mouse RGM‑A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2458) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to retina and developing neurons in cross-section of the developing eye. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

Mouse repulsive guidance molecule (RGM-A) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 454 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 122 aa N-terminal prosegment, a 258 mature region and a 27 aa C-terminal prosegment (2, 3). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of mouse RGM-A is 93% and 87% aa identical to human and chick mature region RGM-A, respectively. When compared to mouse RGMb and c, the mature region of mouse RGM-A shows 77% and 76% aa identity, respectively (2, 3). Recombinant chick RGM has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mice, however, this activity is not so obvious, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure (3). And in a mouse culture system, chick RGM-A is reported to be responsible for the layered segmentation of of entorhinal cortical projections to the hippocampus (4). The receptor for RGM is reported to be neogenin (5, 6).

References

Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
Niederkofler V. et al. (2004) J. Neurosci. 24:808.
Brinks, H. et al. (2004) J. Neurosci. 24:3862.
Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.

Long Name

Repulsive Guidance Molecule A

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Alternate Names

RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse RGM-A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Repulsive guidance molecule a (RGMa) induces neuropathological and behavioral changes that closely resemble Parkinson's disease
Authors: JA Korecka, EB Moloney, R Eggers, B Hobo, S Scheffer, N Ras-Verloo, RJ Pasterkamp, DF Swaab, AB Smit, RE Van Kester, K Bossers, J Verhaagen
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot