Mouse RGM-A Antibody Summary
Accession # Q6PCX7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
RGM‑A in Mouse Embryo. RGM-A was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c) using Goat Anti-Mouse RGM-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2458) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to retina and developing neurons in cross-section of the developing eye. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse repulsive guidance molecule (RGM-A) is a 33 kDa GPI-linked member of an expanding RGM-related family of neuronal and muscle-expressed membrane proteins (1). It is synthesized as a 454 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 122 aa N-terminal prosegment, a 258 mature region and a 27 aa C-terminal prosegment (2, 3). The N-terminal prosegment contains an RGD tripeptide and the molecule’s only two potential N-linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid-proline bond, creating a predicted 32 kDa mature region (2). The mature region of mouse RGM-A is 93% and 87% aa identical to human and chick mature region RGM-A, respectively. When compared to mouse RGMb and c, the mature region of mouse RGM-A shows 77% and 76% aa identity, respectively (2, 3). Recombinant chick RGM has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mice, however, this activity is not so obvious, and thus its function in this system is uncertain (3). Alternatively, in mice, RGM-A is said to be needed for neural tube closure (3). And in a mouse culture system, chick RGM-A is reported to be responsible for the layered segmentation of of entorhinal cortical projections to the hippocampus (4). The receptor for RGM is reported to be neogenin (5, 6).
- Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
- Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
- Niederkofler V. et al. (2004) J. Neurosci. 24:808.
- Brinks, H. et al. (2004) J. Neurosci. 24:3862.
- Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
- Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
Citation for Mouse RGM-A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Repulsive guidance molecule a (RGMa) induces neuropathological and behavioral changes that closely resemble Parkinson's disease
Authors: JA Korecka, EB Moloney, R Eggers, B Hobo, S Scheffer, N Ras-Verloo, RJ Pasterkamp, DF Swaab, AB Smit, RE Van Kester, K Bossers, J Verhaagen
J. Neurosci., 2017;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
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