Mouse S1P5/EDG-8 PE-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB9084P-025
Detection  of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and  eGFP by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse S1P5/EDG-8 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P5/EDG-8 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1196A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse S1P5/EDG-8 peptide corresponding to the N-terminal extracellular sequence
Accession # Q91X56
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and  eGFP by Flow Cytometry. View Larger

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP were stained with either (A) Rabbit Anti-Mouse S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB9084P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P5/EDG-8

S1P5 (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P1 -S1P4 (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P5 is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P5 is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C+ (in human CD14+CD16-) monocytes, pre- and mature oligodendrocytes, and CD27-CD116+ (mature) NK cells. S1P5 appears to play a role in cell trafficking. On monocytes and NK cells, S1P5 promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P5 also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P5 appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P5 shares 96% and 94% aa sequence identity with rat and human S1P5 extracellular domains, respectively.

Long Name
Endothelial Differentiation Gene 8
Entrez Gene IDs
53637 (Human); 94226 (Mouse); 60399 (Rat)
Alternate Names
EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reviews for Mouse S1P5/EDG-8 PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse S1P5/EDG-8 PE-conjugated Antibody and earn rewards!

Have you used Mouse S1P5/EDG-8 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review