Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1196A
View all S1P5/EDG-8 Primary Antibodies »

Product Specifications

Immunogen

Mouse S1P5/EDG-8 peptide corresponding to the N-terminal extracellular sequence
Accession # Q91X56

Specificity

Detects mouse S1P

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse S1P5/EDG‑8 Antibody

Detection of Mouse S1P5/EDG-8 antibody by Western Blot.

Detection of Mouse S1P5/EDG‑8 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mouse S1P5. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse S1P5/EDG-8 Monoclonal Antibody (Catalog # MAB9084) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for S1P5/EDG-8 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of S1P5/EDG-8 antibody in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP antibody by Flow Cytometry.

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP were stained with and either (A) Rabbit Anti-Mouse S1P5/EDG-8 Monoclonal Antibody (Catalog # MAB9084) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse S1P5/EDG‑8 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S1P5/EDG-8

S1P5 (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P1 -S1P4 (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P5 is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P5 is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C+ (in human CD14+CD16-) monocytes, pre- and mature oligodendrocytes, and CD27-CD116+ (mature) NK cells. S1P5 appears to play a role in cell trafficking. On monocytes and NK cells, S1P5 promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P5 also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P5 appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P5 shares 96% and 94% aa sequence identity with rat and human S1P5 extracellular domains, respectively.

Long Name

Endothelial Differentiation Gene 8

Alternate Names

EDG8, LPB4, S1PR5, SPPR-1, SPPR-2

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Gene Symbol

S1PR5

UniProt

Q91X56

Additional S1P5/EDG-8 Products

Product Documents for Mouse S1P5/EDG‑8 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse S1P5/EDG‑8 Antibody

For research use only

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