Mouse S1P5/EDG-8 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9084
MAB9084-SP
Detection  of Mouse S1P5/EDG‑8 by Western Blot.
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Mouse S1P5/EDG-8 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P5/EDG-8 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1196A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse S1P5/EDG-8 peptide corresponding to the N-terminal extracellular sequence
Accession # Q91X56
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Mouse S1P5/EDG‑8 by Western Blot. View Larger

Detection of Mouse S1P5/EDG‑8 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mouse S1P5. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse S1P5/EDG‑8 Monoclonal Antibody
(Catalog # MAB9084) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for S1P5/EDG‑8 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of  S1P5/EDG‑8 in HEK293 Human Cell Line  Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry. View Larger

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP were stained with  and either
(A) Rabbit Anti-Mouse S1P5/EDG‑8 Monoclonal Antibody
(Catalog # MAB9084) or (B) Normal Rabbit IgG Control
(Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P5/EDG-8

S1P5 (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P1 -S1P4 (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P5 is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P5 is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C+ (in human CD14+CD16-) monocytes, pre- and mature oligodendrocytes, and CD27-CD116+ (mature) NK cells. S1P5 appears to play a role in cell trafficking. On monocytes and NK cells, S1P5 promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P5 also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P5 appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P5 shares 96% and 94% aa sequence identity with rat and human S1P5 extracellular domains, respectively.

Long Name
Endothelial Differentiation Gene 8
Entrez Gene IDs
53637 (Human); 94226 (Mouse); 60399 (Rat)
Alternate Names
EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2

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