Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Sema4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Sema4D shares 87% and 93% aa identity with human and rat Sema4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Sema4D binds Plexin-B1, but also CD72 on B and dendritic cells. In developing neurons, Sema-4D assists in guidance. Sema4D is proangiogenic when expressed by tumor-associated macrophages.
Mouse Semaphorin 4D/CD100 Antibody (547412)
R&D Systems | Catalog # MAB52351
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 547412
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100
Phe24-Thr657
Accession # NP_038688
Phe24-Thr657
Accession # NP_038688
Specificity
Detects mouse Semaphorin 4D/CD100 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, or 7A is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Semaphorin 4D/CD100 Antibody (547412)
Detection of Semaphorin 4D/CD100 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Mouse Semaphorin 4D/CD100 Monoclonal Antibody (Catalog # MAB52351, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).Applications for Mouse Semaphorin 4D/CD100 Antibody (547412)
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Semaphorin 4D/CD100
Alternate Names
A8, BB18, CD100, COLL-4, M-sema-G, SEMA4D, SEMAJ
Gene Symbol
SEMA4D
UniProt
Additional Semaphorin 4D/CD100 Products
Product Documents for Mouse Semaphorin 4D/CD100 Antibody (547412)
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Semaphorin 4D/CD100 Antibody (547412)
For research use only
Related Research Areas
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