Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1‑3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that are most like Class 1 invertebrate semaphorins in structure, and exhibit neuropilin-independent binding to specific plexin A receptors (1‑3). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53‑64% within the Sema domain. Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Mouse Sema6C cDNA encodes 931 aa, including a 25 aa signal sequence, a 580 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 305 aa cytoplasmic portion. Alternate exon splicing creates a 923 aa short form (Sema6C.3) that is lacking aa 185‑224 within the Sema domain, but contains 32 unique aa inserted at aa 586; postnatally, this form predominates in muscle (2, 3). The long form predominates in brain, especially in areas of increased plasticity (4). Mouse Sema6C ECD shares 98%, 92%, 92%, 92% and 88% aa identity with corresponding rat, human, porcine, equine and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is downregulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).
Mouse Semaphorin 6C Alexa Fluor® 594‑conjugated Antibody
R&D Systems | Catalog # FAB2108T
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala26-Ile602
Accession # Q9WTM3
Specificity
Clonality
Host
Isotype
Applications for Mouse Semaphorin 6C Alexa Fluor® 594‑conjugated Antibody
Immunohistochemistry
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Semaphorin 6C
Alternate Names
Gene Symbol
UniProt
Additional Semaphorin 6C Products
Product Documents for Mouse Semaphorin 6C Alexa Fluor® 594‑conjugated Antibody
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Product Specific Notices for Mouse Semaphorin 6C Alexa Fluor® 594‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars