Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8224G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TCF7 (Transcription Factor 7), also known as TCF1 (T cell Factor 1) is a member of the Lymphoid Enhancer Binding Factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within amino acids (aa) 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.