Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Antibody

• Species Reactivity
  Mouse
• Specificity
  Detects mouse TCF7/TCF1 in ELISA and Western Blot.
• Source
  Monoclonal Rat IgG_2B Clone # 812145
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant mouse TCF7/TCF1
  Gly61-Gly116
  Accession # Q00417
• Formulation
  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Label
  Alexa Fluor 647

• Intracellular Staining by Flow Cytometry
  0.25-1 µg/10⁶ cells
  See below

Intracellular Staining by Flow Cytometry click image to view larger

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8224R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Shipping
  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
• Stability & Storage
  Protect from light. Do not freeze.

  • 12 months from date of receipt, 2 to 8 °C as supplied.

• Long Name:
  Transcription Factor 7

• Entrez Gene IDs:
  6932 (Human); 21414 (Mouse); 363595 (Rat)

• Alternate Names:
  FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)

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