Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Detects mouse TCF7/TCF1 in ELISA and Western Blot.
Monoclonal Rat IgG2B Clone # 812145
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse TCF7/TCF1
Accession # Q00417
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of TCF7/TCF1 antibody in EL-4 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB8224R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TCF7/TCF1

TCF7 (Transcription factor 7), also called TCF1 (T cell factor 1) is a member of the lymphoid enhancer binding factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within aa 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.

Long Name
Transcription Factor 7
Entrez Gene IDs
6932 (Human); 21414 (Mouse); 363595 (Rat)
Alternate Names
FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)

Product Datasheets

Citations for Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tumor-infiltrating CD62L+PD-1-CD8 T cells retain proliferative potential via Bcl6 expression and replenish effector T cells within the tumor
    Authors: Y Gong, T Suzuki, H Kozono, M Kubo, N Nakano
    PLoS ONE, 2020;15(8):e0237646.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells
    Authors: SS Gupta, R Sharp, C Hofferek, L Kuai, GW Dorn, J Wang, M Chen
    Cell Rep, 2019;29(7):1862-1877.e7.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Flow Cytometry


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