Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8224R-100UG
Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry.
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Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TCF7/TCF1 in ELISA and Western Blot.
Source
Monoclonal Rat IgG2B Clone # 812145
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse TCF7/TCF1
Gly61-Gly116
Accession # Q00417
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. View Larger

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8224R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TCF7/TCF1

TCF7 (Transcription factor 7), also called TCF1 (T cell factor 1) is a member of the lymphoid enhancer binding factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within aa 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.

Long Name
Transcription Factor 7
Entrez Gene IDs
6932 (Human); 21414 (Mouse); 363595 (Rat)
Alternate Names
FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)

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