Mouse TCF7/TCF1 Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TCF7/TCF1 in ELISA and Western Blot.
  • Source
    Monoclonal Rat IgG2B Clone # 812145
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse TCF7/TCF1
    Gly61-Gly116
    Accession # Q00417
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8224R, filled histogram) or isotype control antibody (Catalog # IC013R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TCF7/TCF1
TCF7 (Transcription factor 7), also called TCF1 (T cell factor 1) is a member of the lymphoid enhancer binding factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within aa 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.
  • Long Name:
    Transcription Factor 7
  • Entrez Gene IDs:
    6932 (Human); 21414 (Mouse); 363595 (Rat)
  • Alternate Names:
    FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC013R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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