Mouse TCF7/TCF1 Antibody

• Species Reactivity
  Mouse
• Specificity
  Detects mouse TCF7/TCF1 in ELISA and Western Blot.
• Source
  Monoclonal Rat IgG_2B Clone # 812145
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  E. coli-derived recombinant mouse TCF7/TCF1
  Gly61-Gly116
  Accession # Q00417
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
• Intracellular Staining by Flow Cytometry
  2.5 µg/10⁶ cells
  See below

Western Blot click image to view larger

Detection of Mouse TCF7/TCF1 by Western Blot. Western blot shows lysates of EL‑4 mouse lymphoblast cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TCF7/TCF1 at approximately 30-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry click image to view larger

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Transcription Factor 7

• Entrez Gene IDs:
  6932 (Human); 21414 (Mouse); 363595 (Rat)

• Alternate Names:
  FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)

FAQs

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