Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 812145
View all TCF7/TCF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TCF7/TCF1
Gly61-Gly116
Accession # Q00417

Specificity

Detects mouse TCF7/TCF1 in ELISA and Western Blot.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TCF7/TCF1 Antibody

Detection of Mouse TCF7/TCF1 antibody by Western Blot.

Detection of Mouse TCF7/TCF1 by Western Blot.

Western blot shows lysates of EL-4 mouse lymphoblast cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TCF7/TCF1 at approximately 30-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TCF7/TCF1 antibody in EL-4 Mouse Cell Line antibody by Flow Cytometry.

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry.

EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse TCF7/TCF1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: EL‑4 mouse lymphoblast cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: EL‑4 mouse lymphoblast cell line and mouse thymus tissue

Reviewed Applications

Read 1 review rated 3 using MAB8224 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCF7/TCF1

TCF7 (Transcription factor 7), also called TCF1 (T cell factor 1) is a member of the lymphoid enhancer binding factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within aa 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.

Long Name

Transcription Factor 7

Alternate Names

TCF1

Entrez Gene IDs

6932 (Human); 21414 (Mouse); 363595 (Rat)

Gene Symbol

TCF7

UniProt

Q00417

Additional TCF7/TCF1 Products

Product Documents for Mouse TCF7/TCF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TCF7/TCF1 Antibody

For research use only

Citations for Mouse TCF7/TCF1 Antibody

Customer Reviews for Mouse TCF7/TCF1 Antibody (1)

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  • Mouse TCF7/TCF1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mammary gland tissue
    Species: Mouse
    Verified Customer | Posted 04/20/2019
    Nature of the bands is unclear, but in some instances antibody appears to give bands of the predicted size. 20ug of protein/lane. Dilution 1:1000, blocked with milk in TBST. Appears to work on IF(tissue cryosections)
    Mouse TCF7/TCF1 Antibody MAB8224

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