Mouse TCF7/TCF1 Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse TCF7/TCF1 by Western Blot.
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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Mouse TCF7/TCF1 Antibody Summary

Species Reactivity
Detects mouse TCF7/TCF1 in ELISA and Western Blot.
Monoclonal Rat IgG2B Clone # 812145
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse TCF7/TCF1
Accession # Q00417
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse TCF7/TCF1 antibody by Western Blot. View Larger

Detection of Mouse TCF7/TCF1 by Western Blot. Western blot shows lysates of EL-4 mouse lymphoblast cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TCF7/TCF1 at approximately 30-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of TCF7/TCF1 antibody in EL-4 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF7/TCF1

TCF7 (Transcription factor 7), also called TCF1 (T cell factor 1) is a member of the lymphoid enhancer binding factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within aa 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.

Long Name
Transcription Factor 7
Entrez Gene IDs
6932 (Human); 21414 (Mouse); 363595 (Rat)
Alternate Names
FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)

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Citations for Mouse TCF7/TCF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. From pseudo to real-time dynamics of T cell thymic differentiation
    Authors: Avishai Gavish, Benny Chain, Tomer M. Salame, Yaron E. Antebi, Shir Nevo, Shlomit Reich-Zeliger et al.
  2. A beta -catenin-driven switch in TCF/LEF transcription factor binding to DNA target sites promotes commitment of mammalian nephron progenitor cells
    Authors: Qiuyu Guo, Albert Kim, Bin Li, Andrew Ransick, Helena Bugacov, Xi Chen et al.
  3. Single Extracellular Vesicle Protein Analysis Using Immuno‐Droplet Digital Polymerase Chain Reaction Amplification
    Authors: Jina Ko, Yongcheng Wang, Jonathan C. T. Carlson, Angela Marquard, Jeremy Gungabeesoon, Alain Charest et al.
    Advanced Biosystems


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Mouse TCF7/TCF1 Antibody
By Anonymous on 04/20/2019
Application: WB Sample Tested: Mammary gland tissue Species: Mouse

Nature of the bands is unclear, but in some instances antibody appears to give bands of the predicted size. 20ug of protein/lane. Dilution 1:1000, blocked with milk in TBST.
Appears to work on IF(tissue cryosections)