Mouse TCF7/TCF1 Antibody

Catalog # Availability Size / Price Qty
MAB8224
MAB8224-SP
Mouse TCF7/TCF1 Antibody in Western Blot
2 Images
Product Details
FAQs
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Reviews (1)

Mouse TCF7/TCF1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TCF7/TCF1 in ELISA and Western Blot.
Source
Monoclonal Rat IgG2B Clone # 812145
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse TCF7/TCF1
Gly61-Gly116
Accession # Q00417
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse TCF7/TCF1 by Western Blot. View Larger

Detection of Mouse TCF7/TCF1 by Western Blot. Western blot shows lysates of EL-4 mouse lymphoblast cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TCF7/TCF1 at approximately 30-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. View Larger

Detection of TCF7/TCF1 in EL‑4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse TCF7/TCF1 Monoclonal Antibody (Catalog # MAB8224, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCF7/TCF1

TCF7 (Transcription factor 7), also called TCF1 (T cell factor 1) is a member of the lymphoid enhancer binding factor family of proteins that is expressed in thymocytes and mature T cells. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, the large TCF7/TCF1 isoform (isoform 2, chosen as 'canonical') predominates (419 amino acids, 42‑50 kDa), and serves a transcriptional activator function. The use of an alternate start site at Met116 seems to characterize repressor isoforms. Within aa 61-116 of the large form, mouse TCF7/TCF1 shares 77% aa sequence identity with human TCF7/TCF1.

Long Name
Transcription Factor 7
Entrez Gene IDs
6932 (Human); 21414 (Mouse); 363595 (Rat)
Alternate Names
FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF7; TCF-7; transcription factor 7 (T-cell specific, HMG-box)

Product Datasheets

FAQs

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Reviews for Mouse TCF7/TCF1 Antibody

Average Rating: 3 (Based on 1 Review)

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Mouse TCF7/TCF1 Antibody
By Anonymous on 04/20/2019
Application: WB Sample Tested: Mammary gland tissue Species: Mouse

Nature of the bands is unclear, but in some instances antibody appears to give bands of the predicted size. 20ug of protein/lane. Dilution 1:1000, blocked with milk in TBST.
Appears to work on IF(tissue cryosections)