Mouse TIM-3 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse TIM-3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2A Clone # 215008
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TIM‑3
    Accession # Q8VIM0
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TIM‑3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TIM‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1529G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1 - 3). There are three TIM genes in human and eight in mouse. Mature mouse TIM-3 consists of a 174 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 67 aa cytoplasmic tail (4). Two alternately spliced isoforms have been reported in mouse which lack either the TM or both the TM and mucin regions (5, 6). Within the ECD, mouse TIM-3 shares 58% and 74% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is specifically expressed on Th1 cells whereas TIM-1 and TIM-2 are expressed on TH2 cells. In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types and on hippocampal neurons (9 - 12). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9 which induces TIM-3 Tyr phosphorylation and proapoptotic signaling(10, 13). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation, and cytotoxicity (5, 7, 12, 14). TIM-3 may play a role in regulatory T cell development, (7) inflammation, (15) and immune tolerance (5, 13, 14). Soluble mouse TIM-3 has been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (6, 7).

  • References:
    1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
    2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
    3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
    4. Monney, L. et al. (2002) Nature 415:536.
    5. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
    6. Geng, H. et al. (2006) J. Immunol. 176:1411.
    7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
    8. Khademi, M. et al. (2004) J. Immunol. 172:7169.
    9. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
    10. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
    11. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
    12. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
    13. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
    14. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
    15. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
  • Long Name:
    T Cell Immunoglobulin Mucin-3
  • Entrez Gene IDs:
    84868 (Human); 171285 (Mouse); 102141722 (Cynomolgus Monkey)
  • Alternate Names:
    CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; Tim-3; TIMD-3; TIMD3KIM-3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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