Mouse TLR1 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse TLR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) TLR6 is observed, 5% cross-reactivity with recombinant human (rh) TLR1 and rmTLR2 is observed, and less than 2% cross-reactivity with rhTLR3 and rhTLR4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TLR1
    Accession # Q9EPQ1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TLR1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse TLR1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1475P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least twelve mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature mouse TLR1 consists of a 557 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 192 aa cytoplasmic domain (3, 4). Within the ECD, mouse TLR1 shares 60% aa sequence identity with mouse TLR6 and 19%‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 73% and 86% aa sequence identity with human and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappa B (7, 11, 13).

  • References:
    1. Miyake, K. (2007) Semin. Immunol. 19:3.
    2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
    3. SwissProt # Q9EPQ1.
    4. Matsushima, N. et al. (2007) BMC Genomics 8:124.
    5. Ochoa, M.-T. et al. (2003) Immunology 108:10.
    6. Takeuchi, O. et al. (2002) J. Immunol. 169:10.
    7. Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.
    8. Sandor, F. et al. (2003) J. Cell Biol. 162:1099.
    9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
    10. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.
    11. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
    12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
    13. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
  • Long Name:
    Toll-like Receptor 1
  • Entrez Gene IDs:
    7096 (Human); 21897 (Mouse); 305354 (Rat)
  • Alternate Names:
    CD281 antigen; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012MGC126311; MGC104956; MGC126312; rsc786; TIL; TIL. LPRS5; Toll/interleukin-1 receptor-like protein; toll-like receptor 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse TLR1 PE-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information