Mouse Total MMP-3 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
MMP300
Mouse Total MMP-3 ELISA Standard Curve
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Product Details
Procedure
Citations (8)
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Mouse Total MMP-3 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL)
Sensitivity
0.053 ng/mL
Assay Range
0.3 - 10 ng/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse MMP-3 (pro-, active, and TIMP-complexed)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MMP-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-3 (pro-, active, and TIMP-complexed MMP-3) in cell culture supernates and mouse serum. It contains NS0-expressed recombinant mouse MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant mouse active MMP-3. Natural mouse MMP-3 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.76 3.17 4.72 0.82 3.29 4.99
Standard Deviation 0.04 0.05 0.09 0.08 0.19 0.37
CV% 5.3 1.6 1.9 9.8 5.8 7.4

Recovery

The recovery of mouse MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 92-106
Serum (n=4) 98 93-107

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MMP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse Total MMP-3 ELISA Linearity

Data Examples

Mouse Total MMP-3 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-3

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 3
Entrez Gene IDs:
4314 (Human); 17392 (Mouse)
Alternate Names:
CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse Total MMP-3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Polarizing receptor activation dissociates fibroblast growth factor 2 mediated inhibition of myelination from its neuroprotective potential
    Authors: K Thümmler, E Rom, T Zeis, M Lindner, S Brunner, JJ Cole, D Arseni, S Mücklisch, JM Edgar, N Schaeren-W, A Yayon, C Linington
    Acta Neuropathol Commun, 2019;7(1):212.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Effect of Thrombin-Induced MCP-1 and MMP-3 Production Via PAR1 Expression in Murine Intervertebral Discs
    Authors: Y Takayama, T Ando, J Ichikawa, H Haro
    Sci Rep, 2018;8(1):11320.
    Species: Mouse
    Sample Types: Tissue Supernates
  3. Interleukin-36 receptor mediates the crosstalk between plasma cells and synovial fibroblasts
    Authors: V Schmitt, M Hahn, V Kästele, O Wagner, M Wiendl, A Derer, A Taddeo, S Hahne, A Radbruch, HM Jäck, W Schuh, D Mielenz, S Gay, G Schett, AJ Hueber, S Frey
    Eur. J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Aryl hydrocarbon receptor deficiency in T cells suppresses the development of collagen-induced arthritis.
    Authors: Nakahama T, Kimura A, Nguyen NT, Chinen I, Hanieh H, Nohara K, Fujii-Kuriyama Y, Kishimoto T
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(34):14222-7.
    Species: Mouse
    Sample Types: Serum
  5. Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence.
    Authors: Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA
    J Neuroinflammation, 2011;8(0):115.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. PI3Kgamma regulates cartilage damage in chronic inflammatory arthritis.
    Authors: Hayer S, Pundt N, Peters MA, Wunrau C, Kuhnel I, Neugebauer K, Strietholt S, Zwerina J, Korb A, Penninger J, Joosten LA, Gay S, Ruckle T, Schett G, Pap T
    FASEB J., 2009;23(12):4288-98.
    Species: Mouse
    Sample Types: Serum
  7. Basigin-mediated gene expression changes in mouse uterine stromal cells during implantation.
    Authors: Chen L, Belton RJ, Nowak RA
    Endocrinology, 2009;150(2):966-76.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. The development of cardiac fibrosis in low tissue factor mice is gender-dependent and is associated with differential regulation of urokinase plasminogen activator.
    Authors: Davis DR, Wilson K, Sam MJ, Kennedy SE, Mackman N, Charlesworth JA, Erlich JH
    J. Mol. Cell. Cardiol., 2007;42(3):559-71.
    Species: Mouse
    Sample Types: Tissue Homogenates

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