TREM-3 is a type I transmembrane protein possessing a single extracellular Ig domain. It associates with the signal adapter molecule, DAP12, and delivers an activating signal. TREM-3 mRNA was detected in macrophage cell lines and at low levels in T cell lines, but not in NK, B cell, or mast cell lines.
Mouse TREM‑3 Biotinylated Antibody
R&D Systems | Catalog # BAF2224
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-3
Gly20-Pro135
Accession # NP_067382
Gly20-Pro135
Accession # NP_067382
Specificity
Detects mouse TREM-3 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant mouse (rm) TREM-2b and rmTREM-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse TREM‑3 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TREM‑3
Sample: Recombinant Mouse TREM‑3
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TREM-3
Long Name
Triggering Receptor Expressed on Myeloid Cells 3
Alternate Names
TREM3
Entrez Gene IDs
58218 (Mouse)
Gene Symbol
TREM3
UniProt
Additional TREM-3 Products
Product Documents for Mouse TREM‑3 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TREM‑3 Biotinylated Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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