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Mouse TREML4/TLT-4 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5500R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5500R-100UG

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Mouse TREML4/TLT-4 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREML4/TLT‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREM1, rmTREM2b, and rmTREM3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT‑4
Ser25-Val195
Accession # NP_001029094
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TREML4/TLT-4

TREML4 (Triggering receptor expressed on myeloid cells-like 4; also TLT4) is a 53-55 kDa member of the Ig superfamily.  It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor.  Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length.  It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue.  There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence.  Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

Long Name
Triggering Receptor Expressed on Myeloid Cells-like 4
Entrez Gene IDs
285852 (Human); 224840 (Mouse); 680862 (Rat)
Alternate Names
TLT4; TREML4

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