Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TREML4/TLT-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT‑4
Ser25-Val195
Accession # NP_001029094

Specificity

Detects mouse TREML4/TLT‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREM1, rmTREM2b, and rmTREM3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TREML4/TLT-4 Antibody

Detection of Mouse TREML4/TLT‑4 antibody by Western Blot.

Detection of Mouse TREML4/TLT‑4 by Western Blot.

Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse TREML4/TLT-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5500) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TREML4/TLT-4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse TREML4/TLT-4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse spleen tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TREML4/TLT-4

TREML4 (Triggering receptor expressed on myeloid cells-like 4; also TLT4) is a 53-55 kDa member of the Ig superfamily.  It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor.  Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length.  It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue.  There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence.  Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 4

Alternate Names

TLT4

Entrez Gene IDs

285852 (Human); 224840 (Mouse); 680862 (Rat)

Gene Symbol

TREML4

UniProt

NP_001029094

Additional TREML4/TLT-4 Products

Product Documents for Mouse TREML4/TLT-4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TREML4/TLT-4 Antibody

For research use only

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