Detection of Mouse TREML4/TLT‑4 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse TREML4/TLT‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5500) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TREML4/TLT‑4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TREML4 (Triggering receptor expressed on myeloid cells-like
4; also TLT4) is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, and
based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane
(TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aa
extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the
TM segment contains one Lys residue. There is one splice variant that shows a
deletion of aa 21-24, a segment that lies in the putative signal sequence. Rodent and human TREML4 are highly divergent,
and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat
TREML4, but only 52% aa sequence identity with human TREML4.
Triggering Receptor Expressed on Myeloid Cells-like 4
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