TREML4 (Triggering receptor expressed on myeloid cells-like 4; also TLT4) is a 29 kDa (predicted) member of the Ig superfamily. It is likely expressed by leukocytes and based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane (TM) protein 235 amino acids (aa) in length. It contains a 171 aa extracellular region (aa 29‑199) with a V-type Ig-like domain (aa 29‑122); the TM segment contains one Lys residue. There is one splice variant that shows a deletion of aa 21‑24, a segment that lies in the putative signal sequence. Rodent and human TREML4 are highly divergent and thus over aa 25‑195, mouse TREML4 shares 78% aa identity with rat TREML4, but only 52% aa identity with human TREML4.
Mouse TREML4/TLT‑4 Biotinylated Antibody
R&D Systems | Catalog # BAF5500
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT‑4
Ser25-Val195
Accession # NP_001029094
Ser25-Val195
Accession # NP_001029094
Specificity
Detects mouse TREML4/TLT‑4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TREM1, recombinant mouse (rm) TREM2b, and rmTREM3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse TREML4/TLT‑4 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TREML4/TLT‑4
Sample: Recombinant Mouse TREML4/TLT‑4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TREML4/TLT-4
Long Name
Triggering Receptor Expressed on Myeloid Cells-like 4
Alternate Names
TLT4
Gene Symbol
TREML4
UniProt
Additional TREML4/TLT-4 Products
Product Documents for Mouse TREML4/TLT‑4 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TREML4/TLT‑4 Biotinylated Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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