Mouse Tryptase  epsilon /BSSP‑4 Biotinylated Antibody

R&D Systems | Catalog # BAM20591

R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 246703
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tryptase epsilon /BSSP‑4
Ala33-Ser306
Accession # Q9ER10

Specificity

Detects mouse Tryptase epsilon /BSSP‑4 in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Tryptase  epsilon /BSSP‑4 Biotinylated Antibody

Mouse Tryptase epsilon /BSSP-4 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse Tryptase  epsilon /BSSP‑4 Antibody (Catalog # MAB20592)
  • Standard: Recombinant Mouse Tryptase epsilon/BSSP-4 Protein, CF (Catalog # 2059-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tryptase epsilon/BSSP-4

Tryptase epsilon, brain specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2) are different names given for the same serine protease that is encoded by the PRSS22 gene (1‑3). Initially identified having brain-specific expression, mouse Tryptase epsilon is preferentially expressed in epithelium-rich tissues such as the lung and eye, which is similar to its human counterpart (3). The mouse protein is synthesized with a signal peptide (amino acid residues 1 to 32), a pro peptide (residues 33 to 49) and a mature chain (residues 50 to 306) corresponding to the serine protease domain.

References

  1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
  2. Davies, B.J. et al. (1998) J. Biol. Chem. 273:23004.
  3. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

Alternate Names

BSP-2, BSSP-4, BSSP4, PRSS22

Entrez Gene IDs

64063 (Human); 70835 (Mouse)

Gene Symbol

PRSS22

UniProt

Additional Tryptase epsilon/BSSP-4 Products

Product Documents for Mouse Tryptase  epsilon /BSSP‑4 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Tryptase  epsilon /BSSP‑4 Biotinylated Antibody

For research use only

Related Research Areas

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