Tryptase epsilon, brain specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2) are different names given for the same serine protease that is encoded by the PRSS22 gene (1‑3). Initially identified having brain-specific expression, mouse Tryptase epsilon is preferentially expressed in epithelium-rich tissues such as the lung and eye, which is similar to its human counterpart (3). The mouse protein is synthesized with a signal peptide (amino acid residues 1 to 32), a pro peptide (residues 33 to 49) and a mature chain (residues 50 to 306) corresponding to the serine protease domain.
Mouse Tryptase epsilon /BSSP‑4 Biotinylated Antibody
R&D Systems | Catalog # BAM20591
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Key Product Details
Species Reactivity
Mouse
Applications
ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Monoclonal Rat IgG2A Clone # 246703
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tryptase epsilon /BSSP‑4
Ala33-Ser306
Accession # Q9ER10
Ala33-Ser306
Accession # Q9ER10
Specificity
Detects mouse Tryptase epsilon /BSSP‑4 in ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Tryptase epsilon /BSSP‑4 Biotinylated Antibody
Mouse Tryptase epsilon /BSSP-4 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Tryptase epsilon/BSSP-4
References
- Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
- Davies, B.J. et al. (1998) J. Biol. Chem. 273:23004.
- Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
Alternate Names
BSP-2, BSSP-4, BSSP4, PRSS22
Gene Symbol
PRSS22
UniProt
Additional Tryptase epsilon/BSSP-4 Products
Product Documents for Mouse Tryptase epsilon /BSSP‑4 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Tryptase epsilon /BSSP‑4 Biotinylated Antibody
For research use only
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