Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 657909
View all TSPAN8 Primary Antibodies »

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse TSPAN8

Specificity

Detects mouse TSPAN8 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TSPAN8 APC‑conjugated Antibody

Detection of TSPAN8 antibody in CMT-93 Mouse Cell Line antibody by Flow Cytometry.

Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry.

CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 APC-conjugated Monoclonal Antibody (Catalog # FAB6524A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TSPAN8 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: CMT‑93 mouse rectal carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using FAB6524A in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TSPAN8

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.

Long Name

Tetraspanin 8/Transmembrane 4 Superfamily, Member 3

Alternate Names

CO-029, TM4SF3

Entrez Gene IDs

7103 (Human); 216350 (Mouse); 171048 (Rat)

Gene Symbol

TSPAN8

Additional TSPAN8 Products

Product Documents for Mouse TSPAN8 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TSPAN8 APC‑conjugated Antibody

For research use only

Related Research Areas

Integrins and Associated Molecules

Citations for Mouse TSPAN8 APC‑conjugated Antibody

Customer Reviews for Mouse TSPAN8 APC‑conjugated Antibody (1)

5 out of 5
1 Customer Rating
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  • Mouse TSPAN8 APC-conjugated Antibody
    Name: Anja Kusch
    Application: Flow Cytometry
    Sample Tested: Thymus tissue
    Species: Mouse
    Verified Customer | Posted 02/13/2023
    Gives two clear populations which look very distinct. Clear recommendation.
    Mouse TSPAN8 APC‑conjugated Antibody FAB6524A

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