Mouse TSPAN8 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TSPAN8 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 657909
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CHO Chinese hamster ovary cell line transfected with mouse TSPAN8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT‑93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 APC‑conjugated Monoclonal Antibody (Catalog # FAB6524A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TSPAN8

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.

  • Long Name:
    Tetraspanin 8/Transmembrane 4 Superfamily, Member 3
  • Entrez Gene IDs:
    7103 (Human); 216350 (Mouse); 171048 (Rat)
  • Alternate Names:
    CO-029; tetraspanin 8; TM4SF3; TM4SF3tspan-8; Transmembrane 4 superfamily member 3tetraspanin-8; TSPAN8; Tspan-8; Tumor-associated antigen CO-029
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive
    Authors: NY Fu, AC Rios, B Pal, CW Law, P Jamieson, R Liu, F Vaillant, F Jackling, KH Liu, GK Smyth, GJ Lindeman, ME Ritchie, JE Visvader
    Nat. Cell Biol, 2017;19(3):164-176.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow Cytometery
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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