Mouse TSPAN8 Antibody

Catalog # Availability Size / Price Qty
Detection of TSPAN8 in CMT-93 Mouse Cell Line by Flow Cytometry.
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Product Details
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Mouse TSPAN8 Antibody Summary

Species Reactivity
Detects mouse TSPAN8 transfectants but not the parental cell line in flow cytometry.
Monoclonal Rat IgG2B Clone # 657909
Protein A or G purified from hybridoma culture supernatant
CHO Chinese hamster ovary cell line transfected with mouse TSPAN8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
2.5 µg/106 cells
See below
8-25 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TSPAN8 antibody in CMT-93 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TSPAN8 in CMT-93 Mouse Cell Line by Flow Cytometry. CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524, filled histo-gram) or isotype control antibody (Catalog # MAB0061, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Immunohistochemistry TSPAN8 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

TSPAN8 in Mouse Intestine. TSPAN8 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to membranes of epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN8

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.

Long Name
Tetraspanin 8/Transmembrane 4 Superfamily, Member 3
Entrez Gene IDs
7103 (Human); 216350 (Mouse); 171048 (Rat)
Alternate Names
CO-029; tetraspanin 8; TM4SF3; TM4SF3tspan-8; Transmembrane 4 superfamily member 3tetraspanin-8; TSPAN8; Tspan-8; Tumor-associated antigen CO-029

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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