Mouse TSPAN8 Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TSPAN8 in CMT-93 Mouse Cell Line by Flow Cytometry. CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524, filled histo-gram) or isotype control antibody (Catalog # MAB0061, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
TSPAN8 in Mouse Intestine. TSPAN8 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to membranes of epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.
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