Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 657909
View all TSPAN8 Primary Antibodies »

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse TSPAN8
Accession # Q8R3G9

Specificity

Detects mouse TSPAN8 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TSPAN8 PE‑conjugated Antibody

Detection of TSPAN8 antibody in CMT-93 Mouse Cell Line antibody by Flow Cytometry.

Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry.

CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 PE-conjugated Monoclonal Antibody (Catalog # FAB6524P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TSPAN8 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CMT‑93 mouse rectal carcinoma cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TSPAN8

TSPAN8 (Tetraspanin-8), also known as TM4SF3 and CO-029, is a 27-33 kDa member of the tetraspanin family of proteins. As evidenced by its name, it is a 4-transmembrane (glyco)protein that is found on/in multiple tumor types as well as smooth and skeletal muscle, endothelium and hematopoietic progenitor cells. All 4-transmembrane proteins are not tetraspanins. Tetraspanins are characterized by the presence of a very short intracellular C- and N-terminus, and a very long extracellular (or LEL) domain linking the 3rd and 4th transmembrane segments. On TSPAN8 this domain is 98 amino acids (aa) in length and represents 42% of the entire length of mouse TSPAN8 (or 235 aa). TSPAN8, like other tetraspanins, forms the backbone of a network that allows molecule interactions on (or in) plasma membranes. This ability is dependent upon molecule palmitoylation and creates a microdomain (or TEM) that is distinct from typical membrane rafts, the latter of which is characterized by the presence of GPI-linked proteins and the molecule caveolin. Within this TEM, TSPAN8 may form homodimers, and is known to interact with multiple molecule types, including CD151, CD81, CD9, CD13, EpCAM, EWI-F, MMP-9, Meprin-beta, Rictor, TACE and the integrins alpha 3(CD49c) beta 1, alpha 6 beta 4 and alpha 4(CD49d) beta 1. Such interactions appear to positively or negatively modulate individual molecule activity. These interactions are generally described within the context of exosomes, 30-100 nm membrane-bound vesicles that are released from multiple cell types. TSPAN8-containing exosomes are reported to either facilitate cell motility or act as an antiapoptotic agent for target cells. Over aa 106-203 constituting the LEL, mouse TSPAN8 shares 58% and 73% aa sequence identity with human and rat TSPAN8, respectively.

Long Name

Tetraspanin 8/Transmembrane 4 Superfamily, Member 3

Alternate Names

CO-029, TM4SF3

Entrez Gene IDs

7103 (Human); 216350 (Mouse); 171048 (Rat)

Gene Symbol

TSPAN8

UniProt

Q8R3G9

Additional TSPAN8 Products

Product Documents for Mouse TSPAN8 PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse TSPAN8 PE‑conjugated Antibody

For research use only

Related Research Areas

Integrins and Associated Molecules

Citations for Mouse TSPAN8 PE‑conjugated Antibody

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