Mouse TSPAN8 PE-conjugated Antibody Summary
Accession # Q8R3G9
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 PE-conjugated Monoclonal Antibody (Catalog # FAB6524P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
TSPAN8 (Tetraspanin-8), also known as TM4SF3 and CO-029, is a 27-33 kDa member of the tetraspanin family of proteins. As evidenced by its name, it is a 4-transmembrane (glyco)protein that is found on/in multiple tumor types as well as smooth and skeletal muscle, endothelium and hematopoietic progenitor cells. All 4-transmembrane proteins are not tetraspanins. Tetraspanins are characterized by the presence of a very short intracellular C- and N-terminus, and a very long extracellular (or LEL) domain linking the 3rd and 4th transmembrane segments. On TSPAN8 this domain is 98 amino acids (aa) in length and represents 42% of the entire length of mouse TSPAN8 (or 235 aa). TSPAN8, like other tetraspanins, forms the backbone of a network that allows molecule interactions on (or in) plasma membranes. This ability is dependent upon molecule palmitoylation and creates a microdomain (or TEM) that is distinct from typical membrane rafts, the latter of which is characterized by the presence of GPI-linked proteins and the molecule caveolin. Within this TEM, TSPAN8 may form homodimers, and is known to interact with multiple molecule types, including CD151, CD81, CD9, CD13, EpCAM, EWI-F, MMP-9, Meprin-beta, Rictor, TACE and the integrins alpha 3(CD49c) beta 1, alpha 6 beta 4 and alpha 4(CD49d) beta 1. Such interactions appear to positively or negatively modulate individual molecule activity. These interactions are generally described within the context of exosomes, 30-100 nm membrane-bound vesicles that are released from multiple cell types. TSPAN8-containing exosomes are reported to either facilitate cell motility or act as an antiapoptotic agent for target cells. Over aa 106-203 constituting the LEL, mouse TSPAN8 shares 58% and 73% aa sequence identity with human and rat TSPAN8, respectively.
Citation for Mouse TSPAN8 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive
Authors: NY Fu, AC Rios, B Pal, CW Law, P Jamieson, R Liu, F Vaillant, F Jackling, KH Liu, GK Smyth, GJ Lindeman, ME Ritchie, JE Visvader
Nat. Cell Biol, 2017;19(3):164-176.
Sample Types: Whole Cells
Applications: Flow Cytometry
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