Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT‑93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 PE‑conjugated Monoclonal Antibody (Catalog # FAB6524P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
(Tetraspanin-8), also known as TM4SF3 and CO-029, is a 27-33 kDa member of the
tetraspanin family of proteins. As
evidenced by its name, it is a 4-transmembrane (glyco)protein that is found
on/in multiple tumor types as well as smooth and skeletal muscle, endothelium
and hematopoietic progenitor cells. All
4-transmembrane proteins are not tetraspanins.
Tetraspanins are characterized by the presence of a very short intracellular
C- and N-terminus, and a very long extracellular (or LEL) domain linking the 3rd
and 4th transmembrane segments.
On TSPAN8 this domain is 98 amino acids (aa) in length and represents 42%
of the entire length of mouse TSPAN8 (or 235 aa). TSPAN8, like other tetraspanins, forms the
backbone of a network that allows molecule interactions on (or in) plasma
membranes. This ability is dependent
upon molecule palmitoylation and creates a microdomain (or TEM) that is
distinct from typical membrane rafts, the latter of which is characterized by
the presence of GPI-linked proteins and the molecule caveolin. Within this TEM, TSPAN8 may form homodimers,
and is known to interact with multiple molecule types, including CD151, CD81,
CD9, CD13, EpCAM, EWI-F, MMP-9, Meprin-beta, Rictor, TACE and the integrins alpha 3(CD49c) beta 1, alpha 6 beta 4 and alpha 4(CD49d) beta 1. Such
interactions appear to positively or negatively modulate individual molecule
activity. These interactions are
generally described within the context of exosomes, 30-100 nm membrane-bound
vesicles that are released from multiple cell types. TSPAN8-containing exosomes are reported to
either facilitate cell motility or act as an antiapoptotic agent for target
cells. Over aa 106-203 constituting the
LEL, mouse TSPAN8 shares 58% and 73% aa sequence identity with human and rat
Tetraspanin 8/Transmembrane 4 Superfamily, Member 3
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but also provides information about sample types, species, and experimental conditions.
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