Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2C Clone # 462310
View all Tsukushi/TSK Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tsukushi/TSK
Thr18-Leu354
Accession # Q8CBR6

Specificity

Detects mouse Tsukushi/TSK in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human Tsukushi/TSK is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2C

Applications for Mouse Tsukushi/TSK Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Tsukushi/TSK

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tsukushi/TSK

TSK, also known as Tsukushi and Leucine-rich repeat-containing protein 54, is a member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins, which are characterized by leucine-rich repeats (LRR) flanked by conserved cysteines. Mouse TSK is a secreted glycoprotein that has a predicted molecular weight of 36.5 kDa and contains 9 LLR. Proteoglycan modification of TSK has not been shown. Estrogen treatment in breast cancer cell lines upregulates TSK, and so TSK is proposed to be involved in hormonally regulated extracellular matrix remodeling. Mouse TSK shares 96%, 85%, 51%, and 49% sequence identity with rat, human, chicken, and Xenopus TSK, respectively.

Long Name

Tsukushin Leucine-rich Repeat Containing Protein 54

Alternate Names

E2IG4, LRRC54, TSKU

Entrez Gene IDs

25987 (Human)

Gene Symbol

TSKU

UniProt

Q8CBR6

Additional Tsukushi/TSK Products

Product Documents for Mouse Tsukushi/TSK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Tsukushi/TSK Antibody

For research use only

Related Research Areas

BMP Family

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