Mouse Tsukushi/TSK Antibody

Catalog #: MAB6376 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6376
MAB6376-SP

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All Tsukushi/TSK Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tsukushi/TSK Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Tsukushi/TSK in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human Tsukushi/TSK is observed.

Source

Monoclonal Rat IgG_2C Clone # 462310

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tsukushi/TSK
Thr18-Leu354
Accession # Q8CBR6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Tsukushi/TSK

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tsukushi/TSK

TSK, also known as Tsukushi and Leucine-rich repeat-containing protein 54, is a member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins, which are characterized by leucine-rich repeats (LRR) flanked by conserved cysteines. Mouse TSK is a secreted glycoprotein that has a predicted molecular weight of 36.5 kDa and contains 9 LLR. Proteoglycan modification of TSK has not been shown. Estrogen treatment in breast cancer cell lines upregulates TSK, and so TSK is proposed to be involved in hormonally regulated extracellular matrix remodeling. Mouse TSK shares 96%, 85%, 51%, and 49% sequence identity with rat, human, chicken, and Xenopus TSK, respectively.

Long Name

Tsukushin Leucine-rich Repeat Containing Protein 54

Entrez Gene IDs

25987 (Human)

Alternate Names

E2IG4; E2IG4E2-induced gene 4 protein; leucine rich repeat containing 54; Leucine-rich repeat-containing protein 54; LRRC54; TSK; TSKtsukushi homolog; TSKU; tsukushi small leucine rich proteoglycan homolog (Xenopus laevis); Tsukushi; tsukushi, small leucine rich proteoglycan; tsukushin

Product Datasheets

Product Datasheet

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