Mouse VE-Cadherin APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cadherin-8, rhCadherin-17, rhN-Cadherin, recombinant mouse (rm) E-Cadherin, or rmP-Cadherin is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 162709
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse VE-Cadherin
    Asp46-Gln592 (Gly67 del, Ile69Asp, Lys70Gln)
    Accession # 2208309A
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of VE‑Cadherin in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VE‑Cadherin APC‑conjugated Monoclonal Antibody (Catalog # FAB1002A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VE-Cadherin

The cadherin (Ca++-dependent adherence) superfamily is a large group of membrane-associated glycoproteins that engage in homotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least five major subfamilies based on molecule gene structure, and/or extracellular (EC) and intracellular domains (1-4). Subfamilies include classical/type I, atypical/type II, and desmosomal-related cadherins (1-3). VE-Cadherin (vascular endothelial cadherin; also cadherin-5 and CD144) is a 125 kDa atypical/type II subfamily cadherin. Its subfamily classification is based principally on its genomic structure, as its physical structure is notably divergent from other type II subfamily members (2, 3). Mouse VE-Cadherin is synthesized as a 784 amino acid (aa) type I transmembrane (TM) preproprotein that contains a 24 aa signal peptide, a 21 aa prosequence, a 554 aa extracellular region (ECR), a 21 aa TM segment, and a 164 aa cytoplasmic domain (5, 6). The ECR contains five Ca++-binding cadherin domains that are approximately 105 aa in length. Cadherin domains are comprised of two beta ‑sheets that are oriented like bread in a sandwich. Although complex, the N-terminal cadherin domain mediates trans interactions, while the internal domains contribute to cis multimerizations (7). Mouse VE-Cadherin ECR is 92%, 77%, and 73% aa identical to rat, human and porcine VE-Cadherin ECR, respectively. VE-Cadherin is involved in the maintenance of endothelial permeability. In this regard, VE-Cadherin does not initiate new blood vessel formation; it maintains it once formed. Thus, when VE‑Cadherin is downregulated, cells part and permeability increases (8). Notably, VEGF is known to promote vascular leakage, and apparently does so by inducing a beta ‑arrestin-dependent endocytosis of VE-Cadherin (9). Part of this effect may be mediated by VE‑Cadherin itself which is reported to increase the membrane half-life of VEGF R2 (10). VE-Cadherin acts homotypically at sites of zonula adherens. On each expressing cell, it is proposed that VE-Cadherin first forms a trimer, which then dimerizes with a trimeric counterpart in-trans. Alternatively, two cis-dimers could act in-trans to generate homotypic binding (11). In addition to cell adhesion, VE‑Cadherin also is reported to mediate TGF-beta receptor assembly. When clustered, VE‑Cadherin enhances T beta RII/T beta RI assembly into an active receptor complex on endothelial cells (12). VE-Cadherin is expressed on endothelial cells, trophoblast cells, endothelial progenitor cells and embryonic hematopoietic cells (5, 8, 13, 14).

  • References:
    1. Patel, S.D. et al. (2007) Curr. Opin. Struct. Biol. 13:690.
    2. Vestweber, D. (2008) Arterioscler. Thromb. Vasc. Biol. 28:223.
    3. Vincent, P.A. et al. (2004) Am. J. Physiol. Cell. Physiol. 286:C987.
    4. Cavallaro, U. et al. (2006) Exp. Cell Res. 312:659.
    5. Breier, G. et al. (1996) Blood 87:630.
    6. Huber, P. et al. (1996) Genomics 32:21.
    7. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
    8. Crosby, C.V. et al. (2005) Blood 105:2771.
    9. Gavard, J. and J.S. Gutkind (2006) Nat. Cell Biol. 8:1223.
    10. Calera, M.R. et al. (2004) Exp. Cell Res. 300:248.
    11. Hewat, E.A. et al. (2007) J. Mol. Biol. 365:744.
    12. Rudini, N. et al. (2008) EMBO J. 27:993.
    13. Kogata, N. et al. (2006) Circ. Res. 98:897.
    14. Ema, M. et al. (2006) Blood 108:4018.
  • Long Name:
    Vascular Endothelium Cadherin
  • Entrez Gene IDs:
    1003 (Human); 12562 (Mouse)
  • Alternate Names:
    7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-cadherin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

Ctrl IC013A 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse VE-Cadherin APC-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information