Mouse VEGF R1/Flt-1 Quantikine ELISA Kit

  (26 citations)     
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Assay Procedure
Citations (26)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
  • Sensitivity
    15.2 pg/mL
  • Assay Range
    125.0 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse sVEGF R1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 265 1383 2174 250 1339 2139
Standard Deviation 19 55 69 21 96 134
CV% 7.2 4 3.2 8.4 7.2 6.3

Recovery

The recovery of mouse sVEGF R1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 96 82-110
EDTA Plasma (n=6) 106 92-120
Serum (n=6) 106 96-119
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sVEGF R1 in each matrix were diluted with Calibrator Diluent and then assayed.
 VEGFR1/Flt-1 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGF R1/Flt-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

  • Long Name:
    Vascular Endothelial Growth Factor Receptor 1
  • Entrez Gene IDs:
    2321 (Human); 14254 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Sample Type
  1. NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface
    Authors: SM Blois, N Freitag, I Tirado-Gon, SB Cheng, MM Heimesaat, S Bereswill, M Rose, ML Conrad, G Barrientos, S Sharma
    Sci Rep, 2017;7(1):2189.
    Species: Mouse
    Sample Type: Serum
  2. Motor neurons control blood vessel patterning in the developing spinal cord
    Authors: P Himmels, I Paredes, H Adler, A Karakatsan, R Luck, HH Marti, O Ermakova, E Rempel, ET Stoeckli, C Ruiz de Al
    Nat Commun, 2017;8(0):14583.
    Species: Mouse
    Sample Type: Tissue Homogenates
  3. Renin-Angiotensin System Transgenic Mouse Model Recapitulates Pathophysiology Similar to Human Preeclampsia with Renal Injury that may be Mediated through VEGF
    Authors: J Morgan Denney
    Am. J. Physiol. Renal Physiol, 2016;0(0):ajprenal.0010.
    Species: Mouse
    Sample Type: Plasma
  4. PAD4 Deficiency Decreases Inflammation and Susceptibility to Pregnancy Loss in a Mouse Model
    Authors: Luise Erpenbeck
    Biol. Reprod, 2016;95(6):132.
    Species: Mouse
    Sample Type: Plasma
  5. Reduced Uterine Perfusion Pressure (RUPP) Model of Preeclampsia in Mice
    PLoS ONE, 2016;11(5):e0155426.
    Species: Mouse
    Sample Type: Plasma
  6. Estrogen-related receptor gamma serves a role in blood pressure homeostasis during pregnancy.
    Authors: Luo Y, Kumar P, Chen C, Latham J, Wang L, Tudela C, Alexander J, Shelton J, McKown L, Mendelson C
    Mol Endocrinol, 2015;28(6):965-75.
    Species: Mouse
    Sample Type: Serum
  7. Specific activation of K-RasG12D allele in the bladder urothelium results in lung alveolar and vascular defects.
    Authors: Ayala de la Pena F, Kanasaki K, Kanasaki M, Vong S, Rovira C, Kalluri R
    PLoS ONE, 2014;9(4):e95888.
    Species: Mouse
    Sample Type: Amniotic Fluid
  8. Carbon monoxide prevents hypertension and proteinuria in an adenovirus sFlt-1 preeclampsia-like mouse model.
    Authors: Venditti, Carolina, Casselman, Richard, Young, Iain, Karumanchi, S Ananth, Smith, Graeme N
    PLoS ONE, 2014;9(9):e106502.
    Species: Mouse
    Sample Type: Plasma
  9. Advances in the pathophysiology of pre-eclampsia and related podocyte injury.
    Authors: Craici, Iasmina, Wagner, Steven J, Weissgerber, Tracey L, Grande, Joseph P, Garovic, Vesna D
    Kidney Int, 2014;86(2):275-85.
    Species: Rat
    Sample Type:
  10. Fcgamma receptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies.
    Authors: Justiniano S, Elavazhagan S, Fatehchand K, Shah P, Mehta P, Roda J, Mo X, Cheney C, Hertlein E, Eubank T, Marsh C, Muthusamy N, Butchar J, Byrd J, Tridandapani S
    J Biol Chem, 2013;288(37):26800-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  11. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
    Authors: Suryawanshi A, Mulik S, Sharma S
    J. Immunol., 2011;186(6):3653-65.
    Species: Mouse
    Sample Type: Tissue Homogenates
  12. Role of reactive oxygen species during hypertension in response to chronic antiangiogenic factor (sFlt-1) excess in pregnant rats.
    Authors: Tam Tam KB, Lamarca B, Arany M, Cockrell K, Fournier L, Murphy S, Martin JN, Granger JP
    Am. J. Hypertens., 2011;24(1):110-3.
    Species: Rat
    Sample Type: Plasma
  13. Sera from preeclampsia patients elicit symptoms of human disease in mice and provide a basis for an in vitro predictive assay.
    Authors: Kalkunte S, Boij R, Norris W
    Am. J. Pathol., 2010;177(5):2387-98.
    Species: Mouse
    Sample Type: Serum
  14. Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization.
    Authors: Mazzone M, Dettori D, Leite de Oliveira R, Loges S, Schmidt T, Jonckx B, Tian YM, Lanahan AA, Pollard P, Ruiz de Almodovar C, De Smet F, Vinckier S, Aragones J, Debackere K, Luttun A, Wyns S, Jordan B, Pisacane A, Gallez B, Lampugnani MG, Dejana E, Simons M, Ratcliffe P, Maxwell P, Carmeliet P
    Cell, 2009;136(5):839-51.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  15. Angiotensin receptor agonistic autoantibodies induce pre-eclampsia in pregnant mice.
    Authors: Zhou CC, Zhang Y, Irani RA, Zhang H, Mi T, Popek EJ, Hicks MJ, Ramin SM, Kellems RE, Xia Y
    Nat. Med., 2008;14(8):855-62.
    Species: Mouse
    Sample Type: Plasma
  16. Novel role for vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.
    Authors: Poesen K, Lambrechts D, Van Damme P, Dhondt J, Bender F, Frank N, Bogaert E, Claes B, Heylen L, Verheyen A, Raes K, Tjwa M, Eriksson U, Shibuya M, Nuydens R, Van Den Bosch L, Meert T, D'Hooge R, Sendtner M, Robberecht W, Carmeliet P
    J. Neurosci., 2008;28(42):10451-9.
    Species: Rat
    Sample Type: Cell Lysates
  17. (Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system.
    Authors: Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S
    Am. J. Pathol., 2008;173(6):1911-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
  18. Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin.
    Authors: Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Ohno S, Oike Y, Ishida S
    Invest. Ophthalmol. Vis. Sci., 2008;49(4):1679-85.
    Species: Mouse
    Sample Type: Tissue Homogenates
  19. An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization.
    Authors: Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA
    FASEB J., 2008;22(8):2775-83.
    Species: Mouse
    Sample Type: Tissue Homogenates
  20. Soluble vascular endothelial growth factor receptor-1 protects mice in sepsis.
    Authors: Tsao PN, Chan FT, Wei SC, Hsieh WS, Chou HC, Su YN, Chen CY, Hsu WM, Hsieh FJ, Hsu SM
    Crit. Care Med., 2007;35(8):1955-60.
    Species: Mouse
    Sample Type: Plasma
  21. Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
    Authors: Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H474-81.
    Species: Rat
    Sample Type: Cell Culture Supernates
  22. Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts.
    Authors: Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T
    Am. J. Physiol. Heart Circ. Physiol., 2006;291(3):H1290-8.
    Species: Rat
    Sample Type: Tissue Homogenates
  23. Blockade of VEGF accelerates proteinuria, via decrease in nephrin expression in rat crescentic glomerulonephritis.
    Authors: Hara A, Wada T, Furuichi K, Sakai N, Kawachi H, Shimizu F, Shibuya M, Matsushima K, Yokoyama H, Egashira K, Kaneko S
    Kidney Int., 2006;69(11):1986-95.
    Species: Transgenic Rat
    Sample Type: Urine
  24. Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor.
    Authors: Nagai N, Noda K, Urano T, Kubota Y, Shinoda H, Koto T, Shinoda K, Inoue M, Shiomi T, Ikeda E, Tsubota K, Suda T, Oike Y, Ishida S
    Invest. Ophthalmol. Vis. Sci., 2005;46(3):1078-84.
    Species: Mouse
    Sample Type: Tissue Homogenates
  25. Transduction of soluble Flt-1 gene to peritoneal mesothelial cells can effectively suppress peritoneal metastasis of gastric cancer.
    Authors: Sako A, Kitayama J, Koyama H, Ueno H, Uchida H, Hamada H, Nagawa H
    Cancer Res., 2004;64(10):3624-8.
    Species: Mouse
    Sample Type: Peritoneal Fluid
  26. Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.
    Authors: Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA
    J. Clin. Invest., 2003;111(5):649-58.
    Species: Rat
    Sample Type: Plasma
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