MPP1 Antibody (1C3-1D11) - Azide and BSA Free

Novus Biologicals | Catalog # H00004354-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C3-1D11

Format

Azide and BSA Free
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Product Specifications

Immunogen

MPP1 (AAH02392, 1 a.a. ~ 466 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY

Specificity

MPP1 (1C3-1D11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MPP1 Antibody (1C3-1D11) - Azide and BSA Free

Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02]

Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02]

Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02] - Analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M02), clone 1C3-1D11.Lane 1: MPP1 transfected lysate(52.3 KDa).Lane 2: Non-transfected lysate.
Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02]

Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02]

Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02] - Analysis of MPP1 over-expressed 293 cell line, cotransfected with MPP1 Validated Chimera RNAi ( Cat # H00004354-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MPP1 monoclonal antibody (M02), clone 1C3-1D11 (Cat # H00004354-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02]

Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02]

Western Blot: MPP1 Antibody (1C3-1D11) [H00004354-M02] - MPP1 monoclonal antibody (M02), clone 1E11-1G11 Analysis of MPP1 expression in K-562.
Sandwich ELISA: MPP1 Antibody (1C3-1D11) [H00004354-M02] - Detection limit for recombinant GST tagged MPP1 is approximately 3ng/ml as a capture antibody.

Applications for MPP1 Antibody (1C3-1D11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MPP1

Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.

Alternate Names

55 kDa erythrocyte membrane protein, AAG12, EC 2.7.11, Membrane protein, palmitoylated 1, membrane protein, palmitoylated 1, 55kDa, MRG1, palmitoylated erythrocyte membrane protein, PEMPpalmitoylated 1 (55kD)

Entrez Gene IDs

4354 (Human)

Gene Symbol

MPP1

OMIM

305360 (Human)

Additional MPP1 Products

Product Documents for MPP1 Antibody (1C3-1D11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MPP1 Antibody (1C3-1D11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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