MRGPRF Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-14410

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (94%), Canine (94%), Primate (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all MRGPRF Primary Antibodies »

Product Specifications

Immunogen

Synthetic 18 amino acid peptide from N-Terminus of human MRGPRF.

Epitope

N-Terminus

Reactivity Notes

Expected reactivity based on percent sequence identity: Mouse, Rat, Hamster, Porcine (88%)

Specificity

Human MRGPRF. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for MRGPRF Antibody - BSA Free

Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP3-14410]

Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP3-14410]

Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP3-14410] - MRGPRF antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for MRGPRF Antibody - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: MRGPRF

MRGF is an orphan receptor that may bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain.

Long Name

Mas-related G Protein-coupled Receptor Member F

Alternate Names

GPR140, GPR168, RTA

Gene Symbol

MRGPRF

Additional MRGPRF Products

Product Documents for MRGPRF Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MRGPRF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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