MSH3 Antibody (CL14064) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43856

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14064

Format

Azide and BSA Free
View all MSH3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P20585, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MSH3 Antibody (CL14064) - Azide and BSA Free

MSH3 Antibody (CL14064) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: MSH3 Antibody [NBP3-43856]

Immunocytochemistry/ Immunofluorescence: MSH3 Antibody [NBP3-43856]

Staining in RH-30 cell line with Anti-MSH3 monoclonal antibody).
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human endometrium shows moderate nuclear positivity in glandular and in stromal cells.
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human cervical cancer shows strong nuclear positivity in tumor cells.
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human colorectal cancer shows moderate to strong nuclear positivity in tumor cells.
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human testis shows no positivity in cells in seminiferous ducts.
MSH3 Antibody (CL14064) - Azide and BSA Free Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP3-43856]

Staining of human uterine cervix shows moderate nuclear positivity in squamous epithelial cells.
MSH3 Antibody (CL14064) - Azide and BSA Free Western Blot: MSH3 Antibody [NBP3-43856]

Western Blot: MSH3 Antibody [NBP3-43856]

Analysis in human cell line U-251 MG.

Applications for MSH3 Antibody (CL14064) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. ICC/IF, PFA and Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MSH3

The function of MSH3 is unknown. It is probably a DNA-repair protein. Defects in MSH3 are a cause of susceptibility to endometrial cancer.

Alternate Names

Divergent upstream protein, DNA mismatch repair protein Msh3, DUC1, DUG, DUPMGC163306, hMSH3, Mismatch repair protein 1, MRP1MGC163308, mutS (E. coli) homolog 3, mutS homolog 3 (E. coli)

Gene Symbol

MSH3

Additional MSH3 Products

Product Documents for MSH3 Antibody (CL14064) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MSH3 Antibody (CL14064) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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