MSH3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19417

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MSH3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MSH3. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

127 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MSH3 Antibody - BSA Free

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human lung cancer. MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417]

Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human colon carcinoma, using MSH3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
MSH3 Antibody

Western Blot: MSH3 Antibody [NBP2-19417] -

Western Blot: MSH3 Antibody [NBP2-19417] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MSH3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MSH3 Antibody [NBP2-19417] -

MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MSH3 stained by MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Scale bar= 10 um.
MSH3 Antibody - BSA Free

Western Blot: MSH3 Antibody [NBP2-19417] -

A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for MSH3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MSH3

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Alternate Names

Divergent upstream protein, DNA mismatch repair protein Msh3, DUC1, DUG, DUPMGC163306, hMSH3, Mismatch repair protein 1, MRP1MGC163308, mutS (E. coli) homolog 3, mutS homolog 3 (E. coli)

Gene Symbol

MSH3

UniProt

P20585 (Human)

Additional MSH3 Products

Product Documents for MSH3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MSH3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MSH3 Antibody - BSA Free

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Protocols

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FAQs

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