MST2/STK3 Antibody (24GB4010)

Novus Biologicals | Catalog # NBP3-50766

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB4010
View all MST2/STK3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human MST2/STK3 (Uniprot: Q13188)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MST2/STK3 Antibody (24GB4010)

MST2/STK3 Antibody (24GB4010)

Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody (24GB4010)[NBP3-50766] -

Immunocytochemical staining of HepG2 cells with MST2/STK3 antibody (1:1,000). Nuclei were stained blue with DAPI; MST2/STK3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
MST2/STK3 Antibody (24GB4010)

Flow Cytometry: MST2/STK3 Antibody (24GB4010)[NBP3-50766] -

Flow cytometric analysis of MST2/STK3expression in HepG2 cells using MST2/STK3 antibody (1:2,000). Green, isotype control; red, STK3.
MST2/STK3 Antibody (24GB4010)

Western Blot: MST2/STK3 Antibody (24GB4010)[NBP3-50766] -

Western blotting analysis using MST2/STK3 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for MST2/STK3 Antibody (24GB4010)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MST2/STK3

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody (24GB4010)

Certificate of Analysis

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Product Specific Notices for MST2/STK3 Antibody (24GB4010)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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