Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MTA1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human Metastasis Associated Protein 1 using the numbering given in Swiss-Prot entry Q13330 (GeneID 9112).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTA1 Antibody - BSA Free

Western Blot: MTA1 Antibody [NB600-260]

Western Blot: MTA1 Antibody [NB600-260]

Western Blot: MTA1 Antibody [NB600-260] - Detection of Human and Mouse MTA1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTA1 antibody NB600-260 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: MTA1 Antibody [NB600-260]

Immunohistochemistry-Paraffin: MTA1 Antibody [NB600-260]

Immunohistochemistry-Paraffin: MTA1 Antibody [NB600-260] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- MTA1 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
Immunoprecipitation: MTA1 Antibody [NB600-260]

Immunoprecipitation: MTA1 Antibody [NB600-260]

Immunoprecipitation: MTA1 Antibody [NB600-260] - Detection of human MTA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTA1 antibody NB600-260 (lot NB600-260-2) used for IP at 6 ug per reaction. MTA1 was also immunoprecipitated by rabbit anti-MTA1 antibody NB600-260-1. For blotting immunoprecipitated MTA1, NB600-260 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for MTA1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MTA1

This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.

Alternate Names

metastasis associated 1, metastasis associated gene 1 protein, metastasis associated protein, metastasis-associated protein MTA1

Entrez Gene IDs

9112 (Human)

Gene Symbol

MTA1

UniProt

Q13330 (Human)

Additional MTA1 Products

Product Documents for MTA1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MTA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MTA1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MTA1 Antibody - BSA Free

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  • Q: We are interested in doing westerns and IPs for MTA-1 (antibody cat# NB600-260) but C. elegans are not listed under species reactivity. Would we be able to get a small aliquot to sample whether it will work for us before making a purchase?

    A: We do not provide free samples, but we do offer our Innovators Reward Program for investigators who would like to test our antibodies in new species or applications.

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