mtTFA Antibody (4H2M9)

Novus Biologicals | Catalog # NBP3-16154

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4H2M9 expressed in HEK293
View all mtTFA Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human mtTFA (NP_003192.1). QDAYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for mtTFA Antibody (4H2M9)

mtTFA Antibody (4H2M9)

Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154] -

Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154] - Western blot analysis of various lysates, using TmtTFA Rabbit mAb at 1:1200 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
mtTFA Antibody (4H2M9)

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] -

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] - Confocal imaging of paraffin-embedded Human colon cancer tissue using mtTFA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
mtTFA Antibody (4H2M9)

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] -

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] - Confocal imaging of paraffin-embedded Human kidney tissue using mtTFA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
mtTFA Antibody (4H2M9)

Immunohistochemistry: mtTFA Antibody (4H2M9) [NBP3-16154] -

Immunohistochemistry: mtTFA Antibody (4H2M9) [NBP3-16154] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using mtTFA Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
mtTFA Antibody (4H2M9)

Immunohistochemistry: mtTFA Antibody (4H2M9) [NBP3-16154] -

Immunohistochemistry: mtTFA Antibody (4H2M9) [NBP3-16154] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using mtTFA Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
mtTFA Antibody (4H2M9)

Immunohistochemistry: mtTFA Antibody (4H2M9) [NBP3-16154] -

Immunohistochemistry: mtTFA Antibody (4H2M9) [NBP3-16154] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using mtTFA Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for mtTFA Antibody (4H2M9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mtTFA

TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.

Alternate Names

mitochondrial transcription factor A, MTTF1, mtTFA, TCF-6, TCF6L1, TCF6L2Mitochondrial transcription factor 1, TCF6TCF6L3, Transcription factor 6, Transcription factor 6-like 2, Transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor A, mitochondrial

Gene Symbol

TFAM

Additional mtTFA Products

Product Documents for mtTFA Antibody (4H2M9)

Certificate of Analysis

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Product Specific Notices for mtTFA Antibody (4H2M9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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