MUC4 Antibody (5J3R7)

Novus Biologicals | Catalog # NBP3-16186

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J3R7 expressed in HEK293
View all MUC-4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 4807-4916 of human MUC4 (Q99102). WATVSVIALSNILHASASLPPEYQNRTEGLLGVWNNNPEDDFRMPNGSTIPPGSPEEMLFHFGMTWQINGTGLLGKRNDQLPSNFTPVFYSQLQKNSSWAEHLISNCDGD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MUC4 Antibody (5J3R7)

MUC4 Antibody (5J3R7)

Western Blot: MUC4 Antibody (5J3R7) [NBP3-16186] -

Western Blot: MUC4 Antibody (5J3R7) [NBP3-16186] - Western blot analysis of various lysates using MUC4 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186] - Immunohistochemistry of paraffin-embedded human colon using MUC4 Rabbit mAb (NBP3-16186) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186]

Immunohistochemistry-Paraffin: MUC4 Antibody (5J3R7) [NBP3-16186] - Immunohistochemistry of paraffin-embedded rat kidney using MUC4 Rabbit mAb (NBP3-16186) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for MUC4 Antibody (5J3R7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MUC-4

MUC4 is a constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, and is a highly glycosylated proteins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. MUC4 antibody studies have shown that MUC4 encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of MUC4 have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. MUC4 is expressed in a wide variety of carcinomas making MUC4 antibodies a key cancer research tool.

Long Name

Mucin 4, Cell Surface-associated

Alternate Names

ASGP, MUC4

Gene Symbol

MUC4

Additional MUC-4 Products

Product Documents for MUC4 Antibody (5J3R7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MUC4 Antibody (5J3R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MUC4 Antibody (5J3R7)

There are currently no reviews for this product. Be the first to review MUC4 Antibody (5J3R7) and earn rewards!

Have you used MUC4 Antibody (5J3R7)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies