Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Monkey (90%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Mucolipin 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of mouse Mucolipin 1 (within residues 500-580). [Swiss-Prot# Q99J21]

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34964698).

Localization

Cell membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mucolipin 1 Antibody - BSA Free

Western Blot: Mucolipin 1 AntibodyBSA Free [NB110-82375]

Western Blot: Mucolipin 1 AntibodyBSA Free [NB110-82375]

Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
Simple Western: Mucolipin 1 AntibodyBSA Free [NB110-82375]

Simple Western: Mucolipin 1 AntibodyBSA Free [NB110-82375]

Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Mucolipin 1 Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:40

Western Blot

0.1-0.5 ug/ml
Application Notes

This Mucolipin 1 antibody is useful for Western blot, where a band can be seen at approx. 66 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:40, apparent MW was 55 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 4 using NB110-82375 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mucolipin 1

Mucolipin 1 (MCOLN1, mucolipidin or TRPML1), is a member of TRP ion channels family and unlike most TRPs, mucolipin 1 primarily resides in the membranes of endocytic pathway (late endosomes/lysosomes). Widely expressed as multipass membrane protein, mucolipin 1's high expression levels are found in brain, liver and kidney. Mucolipin 1 activation by phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P(2)], which is predominantly found in endocytic pathway and mucolipin 1 regulates the fusion/fission of endocytic pathway vesicles, and/or some aspect of lysosomal ion homeostasis such as pH, iron, or zinc content. It is a cation channel, and besides controlling membrane trafficking of proteins and lipids, it plays a major role in Ca2+ transport regulating lysosomal exocytosis. MCOLN1 mutation mediated mucolipin 1 down-regulation results in rare lysosomal storage disease mucolipidosis type IV (MLIV) that is characterized by progressive neurodegeneration with early onset, mental retardation, corneal opacity, retinal degeneration, vacuolization of parietal cells, constitutive achlorhydria and an elevated gastrin levels.

Long Name

Mucolipin-1

Alternate Names

MCOLN1, MG-2, ML1, ML4, MSTP080, Mucolipidin, TRPML1

Entrez Gene IDs

57192 (Human); 94178 (Mouse); 288371 (Rat)

Gene Symbol

MCOLN1

UniProt

Q99J21 (Mouse)

Additional Mucolipin 1 Products

Product Documents for Mucolipin 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mucolipin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mucolipin 1 Antibody - BSA Free

Customer Reviews for Mucolipin 1 Antibody - BSA Free (1)

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  • Name: Math Cuajungco
    Application: Western Blot
    Sample Tested: HEK lysate overexpressing mcoln1
    Species: Mouse
    Verified Customer | Posted 06/08/2011

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Protocols

View specific protocols for Mucolipin 1 Antibody - BSA Free (NB110-82375):

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

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FAQs for Mucolipin 1 Antibody - BSA Free

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  • Q: Has the Mucolipin 1 Antibody (NB110-82375) been used in any publications?

    A: At this time NB110-82375 has not been cited in any publications, however a customer has provided a positive review for this product which you can view on the Images, Reviews & Publications tab of the product datasheet.

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