Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647]

Novus Biologicals | Catalog # NBP3-08534AF647

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Reptile

Applications

Immunohistochemistry-Paraffin, Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # PLP1/4259
View all Myelin PLP Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide GRGTKF that recognizes the C terminal region (aa272-277) of Myelin PLP protein. (Uniprot: P60201)

Reactivity Notes

Tenerife Lizard

Localization

Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647]

Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647]

Flow Cytometry: Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] [NBP3-08534AF647]

Flow Cytometry: Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] [NBP3-08534AF647] - Total extracellular vesicles with Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] (left). Gate determined vesicles positive for PLP1 antibody ranging from 500-800nm size. Image on the right represents the same sample without antibody. 10ul of pre-isolated extracellular vesicles were incubated with 2ul of Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647], during 20 minutes at room temperature, and analyzed in nanoscale flow cytometer able to resolve particles scattering as little as 100um. Image from a verified customer review.

Applications for Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647]

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP3-08534AF647 in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Myelin PLP

Human PLP (also known as lipophilin) is a 30 kDa protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in compaction. Different mutations of the human PLP gene product result in two neurological disorders - Pelizaeus-Merzbacher disease and Spastic Paraplegia type 2.

Long Name

Proteolipid Protein

Alternate Names

DM-20, Lipophilin, MMPL, PLP1, SPG2

Gene Symbol

PLP1

Additional Myelin PLP Products

Product Documents for Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647]

Certificate of Analysis

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Product Specific Notices for Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

Customer Reviews for Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] (1)

4 out of 5
1 Customer Rating
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Customer Images

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  • Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647]
    Name: Matheus Uba Chupel
    Application: Flow Cytometry
    Sample Tested: Human plasma exosomes
    Species: Human
    Verified Customer | Posted 09/23/2024
    Total extracellular vesicles with PLP1-AF647 antibody (left). Gate determined vesicles positive for PLP1 antibody ranging from 500-800nm size. Image on the right represents the same sample without antibody.
    10ul of pre-isolated extracellular vesicles were incubated with 2ul of PLP1-AF647 antibody, during 20 minutes at room temperature, and analyzed in nanoscale flow cytometer able to resolve particles scattering as little as 100um. Despite some background noise possibly given to antibody aggregation (common in such sensitive application), the antibody performed well to detect PLP1+exosomes.
    Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] NBP3-08534AF647
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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