Myeloperoxidase/MPO Antibody (2C7) - BSA Free

Novus Biologicals | Catalog # NB100-64803

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C7

Format

BSA Free
View all Myeloperoxidase/MPO Primary Antibodies »

Product Specifications

Immunogen

Human myeloperoxidase

Reactivity Notes

Predicted cross-reactivities: Dog

Specificity

NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Myeloperoxidase/MPO Antibody (2C7) - BSA Free

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.
Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - An intracellular stain was performed on Jurkat cells with Myeloperoxidase antibody (2C7) NB100-64803PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.
Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm.
Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803]

Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Analysis using the FITC conjugate of NB100-64803. Staining of permeabilised human peripheral blood granulocytes.

Applications for Myeloperoxidase/MPO Antibody (2C7) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000-1:5000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:100-1:2000
Application Notes
Membrane permeabilisation is required for Flow Cytometry. 2C7 recognises myeloperoxidase under nonreducing conditions.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myeloperoxidase/MPO

Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.

Alternate Names

MPO

Gene Symbol

MPO

UniProt

P05164

Additional Myeloperoxidase/MPO Products

Product Documents for Myeloperoxidase/MPO Antibody (2C7) - BSA Free

Certificate of Analysis

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Product Specific Notices for Myeloperoxidase/MPO Antibody (2C7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Myeloperoxidase/MPO Antibody (2C7) - BSA Free

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Protocols

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FAQs for Myeloperoxidase/MPO Antibody (2C7) - BSA Free

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  • Q: I am trying to create an MPO-DNA sandwich ELISA for immune complexes and I have been unsuccessful so far. I have been using a mouse polyclonal anti-human MPO antibody to coat, but I think this is the problem. I am interested in your monoclonal MPO Ab and think it might work but I can't afford to buy a full tube and have it not work for my experiment. I was wondering if there is any way you can send a small aliquot to test one of my samples? If it works then I would be interested in purchasing. I hope you understand and can help in some way.

    A: Unfortunately, it is company policy that we do not send out free samples, as we have a 100% guarantee on all of our products for the applications and species listed on the datasheet. Yes, we recommend using a monoclonal as the capture antibody in ELISA and NB100-64803 is absolutely recommended for this.

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