MYL4 Antibody (OTI1H6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72856

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1H6

Format

Azide and BSA Free
View all MYL4 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 2-197 of human MYL4(NP_002467) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MYL4 Antibody (OTI1H6) - Azide and BSA Free

Western Blot: MYL4 Antibody (OTI1H6)Azide and BSA Free [NBP2-72856]

Western Blot: MYL4 Antibody (OTI1H6)Azide and BSA Free [NBP2-72856]

Western Blot: MYL4 Antibody (OTI1H6) - Azide and BSA Free [NBP2-72856] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL4.
Western Blot: MYL4 Antibody (OTI1H6)Azide and BSA Free [NBP2-72856]

Western Blot: MYL4 Antibody (OTI1H6)Azide and BSA Free [NBP2-72856]

Western Blot: MYL4 Antibody (OTI1H6) - Azide and BSA Free [NBP2-72856] - Analysis of extracts (10ug) from 3 different cell lines and 2 human tissues.

Applications for MYL4 Antibody (OTI1H6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYL4

Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

ALC1, AMLC, GT1, MLC1, Myosin light chain 1, embryonic muscle/atrial isoform, myosin light chain 4, Myosin light chain alkali GT-1 isoform, myosin, atrial/fetal muscle, light chain, myosin, light chain 4, alkali; atrial, embryonic, myosin, light polypeptide 4, alkali; atrial, embryonic, PRO1957

Gene Symbol

MYL4

Additional MYL4 Products

Product Documents for MYL4 Antibody (OTI1H6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MYL4 Antibody (OTI1H6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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