MyoD Antibody (5.2F)

Novus Biologicals | Catalog # NB120-788

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5.2F
View all MyoD Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length Mouse MyoD1 protein.

Epitope

This antibody is specific to an epitope of amino acid 3-56 in mouse MyoD1.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

This antibody is specific to a 45 kD protein, which is indenitified as MyoD1. It does not react with myogenin, Myf5 or Myf6. This antibody stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MyoD Antibody (5.2F)

Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]

Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]

Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788] - Formalin fixed paraffin embedded human rhabdomyosarcoma stained with MyoD antibody (NB120-788).
Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]

Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788]

Immunohistochemistry-Paraffin: MyoD Antibody (5.2F) [NB120-788] - Staining of rhabdomyosarcoma.

Applications for MyoD Antibody (5.2F)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500
Application Notes
IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MyoD

MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Entrez Gene IDs

17927 (Mouse)

Gene Symbol

MYOD1

Additional MyoD Products

Product Documents for MyoD Antibody (5.2F)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MyoD Antibody (5.2F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MyoD Antibody (5.2F)

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  • Q: For the following product, could you let us know the buffer composition and the concentration of sodium azide?

    A: Our lab has confirmed that NB120-788 is supplied in PBS with 2% BSA and 0.05% sodium azide.

  • Q: What is the concentration of this product?

    A: The concentration of the antibody for lot A418 is 97.56 ug/ml

  • Q: For the following product, could you let us know the buffer composition and the concentration of sodium azide?

    A: Our lab has confirmed that NB120-788 is supplied in PBS with 2% BSA and 0.05% sodium azide.

  • Q: What is the concentration of this product?

    A: The concentration of the antibody for lot A418 is 97.56 ug/ml

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