Myosin VB Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-87746
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Scientific Data Images for Myosin VB Antibody - BSA Free
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium, placenta, skeletal muscle and small intestine using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein distribution across tissues to independent antibody NBP1-87747 (B).Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.Immunocytochemistry/ Immunofluorescence: Myosin VB Antibody - BSA Free [NBP1-87746] -
TGF-beta increases Proximity ligation signal between Myo5b and Rab11b, and FN1 and Rab11b, respectively. Confocal images of HPMCs with proximity ligation assay. Proximity ligation signals were shown in red. (A) Effect of TGF-beta stimulation on Proximity ligation signal between Myo5b and V5-Rab11b. V5-Rab11b was expressed in HPMCs for 16 h and proximity ligation assay was performed. Endogenous Myo5b and V5-Rab11b were stained with anti-Myo5b- and anti-v5-antibodies, respectively. Left, Representative confocal images. Magnified images of the boxed area are shown in the right panels. Right, Statistical representation (mean +/- SD). *** p < 0.001. (B) Effect of TGF-beta stimulation on Proximity ligation signal between endogenous FN1 and endogenous Rab11b. Endogenous Rab11b and FN1 were stained with specific antibodies, respectively. Left, Representative confocal image. Magnified images of the boxed area are shown in the right panels. Right, Statistical representation. Scale bar; 20 μm. The red spots in bar graphs display the distribution of data points. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35563212), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Myosin VB Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Knockout Validated
Simple Western
Western Blot
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:100, apparent MW was 199 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
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Background: Myosin VB
Alternate Names
Gene Symbol
Additional Myosin VB Products
Product Documents for Myosin VB Antibody - BSA Free
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Product Specific Notices for Myosin VB Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Myosin VB Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars