Myosin VB Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87746

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western

Cited:

Knockout Validated, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Myosin VB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin VB Antibody - BSA Free

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues. Corresponding MYO5B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium, placenta, skeletal muscle and small intestine using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein distribution across tissues to independent antibody NBP1-87747 (B).
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746]

Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.

Applications for Myosin VB Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:100
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:100, apparent MW was 199 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin VB

Myosin and Kinesin motor domain. These ATPases belong to the P-loop NTPase family and provide the driving force in myosin and kinesin mediated processes.

Alternate Names

KIAA1119, MYO5B variant protein, myosin VB, myosin-Vb

Gene Symbol

MYO5B

Additional Myosin VB Products

Product Documents for Myosin VB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Myosin VB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Myosin VB Antibody - BSA Free

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Protocols

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FAQs

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