Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all N-acylmannosamine 1-dehydrogenase Primary Antibodies »

Product Specifications

Immunogen

N-acylmannosamine 1-dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ] (Uniprot: P22441)

Reactivity Notes

Cross reactivity against N-acylmannosamine 1-dehydrogenase from other sources is unknown.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannoseamine-1-Dehydrogenase [Recombinant from Pseudomonas]

Scientific Data Images for N-acylmannosamine 1-dehydrogenase Antibody [Biotin]

Western Blot: N-acylmannosamine 1-dehydrogenase Antibody [Biotin] [NBP1-77708] - Lane 1: N-Acylmannoseamide 1-Dehydrogenase. Load: 50 ng per lane. Primary antibody: Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 27.5 kDa, observed at 30 kDa for N-Acylmannoseamide 1-Dehydrogenase.
Western Blot: N-acylmannosamine 1-dehydrogenase Antibody [Biotin] [NBP1-77708] - Goat anti N-acylmanosamino-1-Dehydrogenase antibody was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat 1:10K in TBS 1 hr RT).
N-acylmannosamine 1-dehydrogenase Antibody [Biotin]

N-acylmannosamine 1-dehydrogenase Antibody [Biotin]

Western Blot of Goat anti-N-Acylmannoseamide 1-Dehydrogenase Antibody Biotin Conjugated. Lane 1: N-Acylmannoseamide 1-Dehydrogenase. Load: 50 ng per lane. Primary antibody: Goat anti-N-acylmannosamine 1-dehydrogenase Antibody [Biotin]1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 27.5 kDa, observed at 30 kDa for N-Acylmannoseamide 1-Dehydrogenase.

Applications for N-acylmannosamine 1-dehydrogenase Antibody [Biotin]

Application
Recommended Usage

ELISA

1:1000-1:5000

Immunoprecipitation

1:100

Western Blot

1:250-1:1000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of N-Acylmannoseamine-1-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:35,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: N-acylmannosamine 1-dehydrogenase

Alternate Names

NAM-DH

UniProt

P22441 (Bacteria)

Additional N-acylmannosamine 1-dehydrogenase Products

Product Documents for N-acylmannosamine 1-dehydrogenase Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for N-acylmannosamine 1-dehydrogenase Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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