N‑Me‑6,7‑diOH‑TIQ Alexa Fluor™ Plus 680‑conjugated Antibody

Recognizes N‑Me‑6,7‑diOH‑TIQ in immunoassays. The specificity was determined by a competition type assay where the N‑Me‑6,7‑diOH‑TIQ labeled with biotin at position 5 or 8 competed with another compound for binding to the antibody binding site. The detection limit of biotinylated N‑Me‑6,7‑diOH‑TIQ in assays where the molecule was captured by the antibody coated on microplates and developed by HRP‑streptavidin was 5 nM. The dissociation constants of the interaction between the antibody and N‑Me‑6,7‑diOH‑TIQ and its biotinylated analog were 0.2 and 0.5 µM, respectively. This antibody specifically recognizes N‑Me‑6,7‑diOH‑TIQ and shows 60% cross‑reactivity with 6,7‑diOH‑TIQ, 15% cross‑reactivity with 1,N‑di‑Me‑6,7‑diOH‑TIQ, 3% cross‑reactivity with 1‑Me‑6,7‑diOH‑TIQ, and less than 1% or no cross‑reactivity with tetrahydroisoquinoline (TIQ), 5‑OH‑isoquiniline, 5‑OH‑N‑Me‑isoquinolinium, tetrahydropapaveroline, 9H‑pyrido[3,4‑b]indole, and 1,2,3,4‑tetrahydro‑9H‑pyrido[3,4‑b]indole. The percent cross‑reactivity is expressed by the relative value, which produced 50% inhibition of interaction between the biotin‑labeled N‑methyl‑6,7‑dihydroxytetrahydroisoquinoline and the other compound.

Monoclonal

Mouse

IgG_2B

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied