Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 50 and 100 of human Nuclear Assembly Factor 1 using the numbering given in entry NP_612395.2 (GeneID 92345).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NAF1 Antibody - BSA Free
Immunoprecipitation: NAF1 Antibody [NBP2-22263]
Immunoprecipitation: NAF1 Antibody [NBP2-22263] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies NBP2-22263 used for IP at 6 ug/mg lysate. NAF1 was also immunoprecipitated by rabbit anti-NAF1 antibody. For blotting immunoprecipitated NAF1, NBP2-22263 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for NAF1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:500 - 1:2500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NAF1
Alternate Names
nuclear assembly factor 1 homolog (S. cerevisiae)
Gene Symbol
NAF1
UniProt
Additional NAF1 Products
Product Documents for NAF1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NAF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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