Natriuretic Peptide Receptor C Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-31365
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Rabbit
Cited:
Human, Mouse
Predicted:
Bovine (95%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot, SDS-Page
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Natriuretic Peptide Receptor C. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (82%).
Localization
Membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Natriuretic Peptide Receptor C Antibody - BSA Free
Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365]
Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] - Mouse testis. NPR-C antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365]
Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] - Mouse duodenum. NPR-C antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] -
Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] - Natriuretic Peptide Receptor C antibody [N3C3] detects Natriuretic Peptide Receptor C protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.
Natriuretic Peptide Receptor C stained by Natriuretic Peptide Receptor C antibody [N3C3] (NBP1-31365) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Natriuretic Peptide Receptor C Antibody - BSA Free [NBP1-31365] -
Modulation of Nprc expression by rosiglitazone and PPAR gamma.A, mRNA levels of Nprc and Fabp4 in 3T3-L1 adipocytes after treatment with 1 μM rosiglitazone (Rosi) or vehicle (Veh) for 6 h. B, mRNA levels of Nprc, Fabp4, and Ppar gamma 1/2 mRNA in 3T3-L1 adipocytes after siRNA knockdown of Ppar gamma (siPpar gamma ). C, protein levels of PPAR gamma 1/2, NPRC, FABP4, and ADIPOQ in 3T3-L1 adipocytes after siPpar gamma. D, mRNA levels of Nprc and Fabp4 in NIH-3T3 cells (NIH) and NIH-3T3 cells stably expressing PPAR gamma (NIH-PPAR gamma ) after treatment with 1 μM rosiglitazone (Rosi) or vehicle (Veh) for 6 h. Quantitative PCR data were normalized with 36B4. Student's t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. Fabp4, fatty acid–binding protein 4; NIH, National Institutes of Health; Nprc, NP receptor C; ns, not significant; PPAR gamma, peroxisome proliferator–activated receptor gamma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34245781), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Natriuretic Peptide Receptor C Antibody - BSA Free [NBP1-31365] -
Nprc mRNA expression during 3T3-L1 adipocyte differentiation.A–C, mRNA levels of Nprc (A), Fabp4 (B), and Ppar gamma 1/2 (C) during 3T3-L1 adipocyte differentiation. Data were normalized with 36B4. D, protein levels of NPRC, FABP4, PPAR gamma 1/2, and ADIPOQ during 3T3-L1 adipocyte differentiation (Day 0, 2, 4, and 6). FABP4, fatty acid–binding protein 4; Nprc, NP receptor C; PPAR gamma 1/2, peroxisome proliferator–activated receptor gamma1/2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34245781), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Natriuretic Peptide Receptor C Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
Assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NPRC/NPR3
Long Name
Natriuretic Peptide Receptor C
Alternate Names
ANP-C, ANPR-C, C5orf23, GUCY2B, lgj, NPR-C, NPR3, stri
Gene Symbol
NPR3
Additional NPRC/NPR3 Products
Product Documents for Natriuretic Peptide Receptor C Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Natriuretic Peptide Receptor C Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for Natriuretic Peptide Receptor C Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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