Natriuretic Peptide Receptor C Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31365

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Rabbit

Cited:

Human, Mouse

Predicted:

Bovine (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot, SDS-Page

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Natriuretic Peptide Receptor C. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (82%).

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Natriuretic Peptide Receptor C Antibody - BSA Free

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365]

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365]

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] - Mouse testis. NPR-C antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365]

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365]

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] - Mouse duodenum. NPR-C antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Natriuretic Peptide Receptor C Antibody

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] -

Immunohistochemistry-Paraffin: Natriuretic Peptide Receptor C Antibody [NBP1-31365] - Natriuretic Peptide Receptor C antibody [N3C3] detects Natriuretic Peptide Receptor C protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Natriuretic Peptide Receptor C stained by Natriuretic Peptide Receptor C antibody [N3C3] (NBP1-31365) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Natriuretic Peptide Receptor C Antibody - BSA Free

Western Blot: Natriuretic Peptide Receptor C Antibody - BSA Free [NBP1-31365] -

Modulation of Nprc expression by rosiglitazone and PPAR gamma.A, mRNA levels of Nprc and Fabp4 in 3T3-L1 adipocytes after treatment with 1 μM rosiglitazone (Rosi) or vehicle (Veh) for 6 h. B, mRNA levels of Nprc, Fabp4, and Ppar gamma 1/2 mRNA in 3T3-L1 adipocytes after siRNA knockdown of Ppar gamma (siPpar gamma ). C, protein levels of PPAR gamma 1/2, NPRC, FABP4, and ADIPOQ in 3T3-L1 adipocytes after siPpar gamma. D, mRNA levels of Nprc and Fabp4 in NIH-3T3 cells (NIH) and NIH-3T3 cells stably expressing PPAR gamma (NIH-PPAR gamma ) after treatment with 1 μM rosiglitazone (Rosi) or vehicle (Veh) for 6 h. Quantitative PCR data were normalized with 36B4. Student's t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. Fabp4, fatty acid–binding protein 4; NIH, National Institutes of Health; Nprc, NP receptor C; ns, not significant; PPAR gamma, peroxisome proliferator–activated receptor gamma. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34245781), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Natriuretic Peptide Receptor C Antibody - BSA Free

Western Blot: Natriuretic Peptide Receptor C Antibody - BSA Free [NBP1-31365] -

Nprc mRNA expression during 3T3-L1 adipocyte differentiation.A–C, mRNA levels of Nprc (A), Fabp4 (B), and Ppar gamma 1/2 (C) during 3T3-L1 adipocyte differentiation. Data were normalized with 36B4. D, protein levels of NPRC, FABP4, PPAR gamma 1/2, and ADIPOQ during 3T3-L1 adipocyte differentiation (Day 0, 2, 4, and 6). FABP4, fatty acid–binding protein 4; Nprc, NP receptor C; PPAR gamma 1/2, peroxisome proliferator–activated receptor gamma1/2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34245781), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Natriuretic Peptide Receptor C Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NPRC/NPR3

The family of natriuretic peptides (see MIM 108780) elicit a number of vascular, renal, and endocrine effects that are important in the maintenance of blood pressure and extracellular fluid volume. These effects are mediated by specific binding of the peptides to cell surface receptors in the vasculature, kidney, adrenal, and brain.[supplied by OMIM]

Long Name

Natriuretic Peptide Receptor C

Alternate Names

ANP-C, ANPR-C, C5orf23, GUCY2B, lgj, NPR-C, NPR3, stri

Gene Symbol

NPR3

Additional NPRC/NPR3 Products

Product Documents for Natriuretic Peptide Receptor C Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Natriuretic Peptide Receptor C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Natriuretic Peptide Receptor C Antibody - BSA Free

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Protocols

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FAQs

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