Q: Is the product NBP2-13642 reactive to fish NCCRP1, specifically carp?

A: The immunogen of NCCRP1 antibody NBP2-13642 is less than 65% homologous to carp, so we would not recommend this antibody for your purposes. However, NCCRP1 antibody NB100-2071 may be suitable, as it has been validated in catfish.

NCCRP1 Antibody (5C.6)

Novus Biologicals | Catalog # NB100-2071

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Product Documents

Discontinued Product

NB100-2071 has been discontinued. View all NCCRP1 products.

Product Specifications
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Rat, Porcine, Bovine, Canine, Equine, Fish

Applications

Western Blot, ELISA, Flow Cytometry, Immunoprecipitation, Inhibition Activity

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # 5C.6

View all NCCRP1 Primary Antibodies »

Product Specifications

Immunogen

Purified NCC cells from catfish.

Reactivity Notes

Tilapia and Catfish.

Specificity

Nonspecific Cytotoxic Cell (5C.6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Applications for NCCRP1 Antibody (5C.6)

Application

Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunoprecipitation

1:10 - 1:500

Western Blot

10 ug/ml

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

0.73 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCCRP1

Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.

Alternate Names

LOC342897, NCCRP-1, nonspecific cytotoxic cell receptor protein 1 homolog, non-specific cytotoxic cell receptor protein 1 homolog, non-specific cytotoxic cell receptor protein 1 homolog (zebrafish)

Entrez Gene IDs

342897 (Human)

Gene Symbol

NCCRP1

UniProt

Q6ZVX7 (Human)

Additional NCCRP1 Products

Product Documents for NCCRP1 Antibody (5C.6)

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Certificate of Analysis

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Product Specific Notices for NCCRP1 Antibody (5C.6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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