NDUFA10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32146

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NDUFA10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFA10. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (99%), Bovine (82%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFA10 Antibody - BSA Free

Western Blot: NDUFA10 Antibody [NBP1-32146]

Western Blot: NDUFA10 Antibody [NBP1-32146]

Western Blot: NDUFA10 Antibody [NBP1-32146] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFA10 antibody [N1C3] diluted at 1:500.
Immunohistochemistry-Paraffin: NDUFA10 Antibody [NBP1-32146]

Immunohistochemistry-Paraffin: NDUFA10 Antibody [NBP1-32146]

Immunohistochemistry-Paraffin: NDUFA10 Antibody [NBP1-32146] - Human colon, using NDUFA10 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: NDUFA10 Antibody [NBP1-32146]

Western Blot: NDUFA10 Antibody [NBP1-32146]

Western Blot: NDUFA10 Antibody [NBP1-32146] - Sample(30 ug of whole cell lysate)A:H1299 B:Raji 10% SDS PAGE, antibody diluted at 1:500.
NDUFA10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NDUFA10 Antibody [NBP1-32146] -

NDUFA10 antibody [N1C3] detects NDUFA10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] (NBP1-32146) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for NDUFA10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-32146 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFA10

The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain.

Alternate Names

10 (42kD), CI-42k, CI-42KD, FLJ21923, MGC5103, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Gene Symbol

NDUFA10

Additional NDUFA10 Products

Product Documents for NDUFA10 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NDUFA10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Customer Reviews for NDUFA10 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Name: Sven Dennerlein
    Application: Western Blot
    Sample Tested: HEK293 cells
    Species: Human
    Verified Customer | Posted 03/05/2014

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Protocols

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FAQs

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