NDUFA7 Antibody

Novus Biologicals | Catalog # NBP2-26161

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NDUFA7 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PKLPVGPSHKLSNN corresponding to internal region according to NP_004992.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NDUFA7 Antibody

Western Blot: NDUFA7 Antibody [NBP2-26161]

Western Blot: NDUFA7 Antibody [NBP2-26161]

Western Blot: NDUFA7 Antibody [NBP2-26161] - Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-26161]

Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-26161]

Immunohistochemistry-Paraffin: NDUFA7 Antibody [NBP2-26161] - Analysis of Human Heart shows mitochondrial staining in cardial fibres. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: NDUFA7 Antibody [NBP2-26161]

Western Blot: NDUFA7 Antibody [NBP2-26161]

Western Blot: NDUFA7 Antibody [NBP2-26161] - Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for NDUFA7 Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 8 ug/ml

Immunohistochemistry-Paraffin

4 - 8 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 15 kDa band observed in human, mouse, rat and porcine heart lysates (calculated MW of 12.6 kDa band according to NP_004992.2). IHC-P: Human heart shows mitochondrial staining in cardial fibres.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NDUFA7

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Alternate Names

B14.5a, CI-B14.5a, complex I B14.5a subunit, Complex I-B14.5a, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5kD, B14.5a), NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NADH-ubiquinone oxidoreductase subunit B14.5a

Entrez Gene IDs

4701 (Human); 66416 (Mouse); 299643 (Rat)

Gene Symbol

NDUFA7

Additional NDUFA7 Products

Product Documents for NDUFA7 Antibody

Certificate of Analysis

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Product Specific Notices for NDUFA7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NDUFA7 Antibody

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Protocols

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