NDUFAF5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15644

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NDUFAF5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFAF5. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (80%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFAF5 Antibody - BSA Free

Western Blot: NDUFAF5 Antibody [NBP2-15644]

Western Blot: NDUFAF5 Antibody [NBP2-15644]

Western Blot: C20orf7 Antibody [NBP2-15644] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: NDUFAF5 Antibody [NBP2-15644]

Immunohistochemistry-Paraffin: NDUFAF5 Antibody [NBP2-15644]

Immunohistochemistry-Paraffin: C20orf7 Antibody [NBP2-15644] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.
NDUFAF5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NDUFAF5 Antibody [NBP2-15644] -

C20orf7 antibody [N3C3] detects C20orf7 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: C20orf7 protein stained by C20orf7 antibody [N3C3] (NBP2-15644) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for NDUFAF5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFAF5

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

bA526K24.2, C20orf7, chromosome 20 open reading frame 7, dJ842G6.1, EC 2.1.1, EC 2.1.1.-, FLJ22324, MGC90272, NADH dehydrogenase (ubiquinone) complex I, assembly factor 5, probable methyltransferase C20orf7, mitochondrial

Gene Symbol

NDUFAF5

UniProt

Q5TEU4 (Human)

Additional NDUFAF5 Products

Product Documents for NDUFAF5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NDUFAF5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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