![Western Blot: NDUFB4 Antibody [NBP2-15035]](https://resources.rndsystems.com/images/products/NDUFB4-Antibody-Western-Blot-NBP2-15035-img0003.jpg "Western Blot: NDUFB4 Antibody [NBP2-15035]")
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Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Full length human NDUFB4 Recombinant protein.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chimpanzee (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NDUFB4 Antibody
Western Blot: NDUFB4 Antibody [NBP2-15035]
Western Blot: NDUFB4 Antibody [NBP2-15035] - Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE; antibody diluted at 1:1000.![Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035]](https://resources.rndsystems.com/images/products/NDUFB4-Antibody-Immunohistochemistry-Paraffin-NBP2-15035-img0002.jpg "Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035]")
Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035]
Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.![Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035]](https://resources.rndsystems.com/images/products/NDUFB4-Antibody-Immunohistochemistry-Paraffin-NBP2-15035-img0001.jpg "Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035]")
Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035]
Immunohistochemistry-Paraffin: NDUFB4 Antibody [NBP2-15035] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry: NDUFB4 Antibody [NBP2-15035] -
Immunohistochemistry: NDUFB4 Antibody [NBP2-15035] - NDUFB4 antibody detects NDUFB4 protein at cytosol on human hepatoma by immunohistochemical analysis.Sample: Paraffin-embedded hepatoma.
NDUFB4 antibody (NBP2-15035) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Applications for NDUFB4 Antibody
Application
Recommended Usage
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NDUFB4
Alternate Names
B15, CI-B15, complex I B15 subunit, Complex I-B15, MGC5105, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4 (15kD, B15), NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NADH-ubiquinone oxidoreductase B15 subunit
Gene Symbol
NDUFB4
UniProt
Additional NDUFB4 Products
Product Documents for NDUFB4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NDUFB4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for NDUFB4 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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